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Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway

Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled receptors that are expressed in the oral cavity and serve to detect substances with bitter tastes in foods and medicines. Recent evidence suggests that Tas2Rs are also expressed extraorally, including in immune cells. How...

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Autores principales: Kobayashi, Daichi, Watarai, Tomoya, Ozawa, Madoka, Kanda, Yasuhiro, Saika, Fumihiro, Kiguchi, Norikazu, Takeuchi, Arata, Ikawa, Masahito, Matsuzaki, Shinsuke, Katakai, Tomoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436316/
https://www.ncbi.nlm.nih.gov/pubmed/36059440
http://dx.doi.org/10.3389/fimmu.2022.973880
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author Kobayashi, Daichi
Watarai, Tomoya
Ozawa, Madoka
Kanda, Yasuhiro
Saika, Fumihiro
Kiguchi, Norikazu
Takeuchi, Arata
Ikawa, Masahito
Matsuzaki, Shinsuke
Katakai, Tomoya
author_facet Kobayashi, Daichi
Watarai, Tomoya
Ozawa, Madoka
Kanda, Yasuhiro
Saika, Fumihiro
Kiguchi, Norikazu
Takeuchi, Arata
Ikawa, Masahito
Matsuzaki, Shinsuke
Katakai, Tomoya
author_sort Kobayashi, Daichi
collection PubMed
description Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled receptors that are expressed in the oral cavity and serve to detect substances with bitter tastes in foods and medicines. Recent evidence suggests that Tas2Rs are also expressed extraorally, including in immune cells. However, the role of Tas2Rs in immune cells remains controversial. Here, we demonstrate that Tas2R126, Tas2R135, and Tas2R143 are expressed in mouse neutrophils, but not in other immune cells such as macrophages or T and B lymphocytes. Treatment of bone marrow-derived neutrophils from wild-type mice with the Tas2R126/143 agonists arbutin and d-salicin led to enhanced C-X-C motif chemokine ligand 2 (CXCL2)-stimulated migration in vitro, but this response was not observed in neutrophils from Tas2r126/135/143-deficient mice. Enhancement of CXCL2-stimulated migration by Tas2R agonists was accompanied by increased phosphorylation of myosin light chain 2 (MLC2) and was blocked by pretreatment of neutrophils with inhibitors of Rho-associated coiled-coil-containing protein kinase (ROCK), but not by inhibitors of the small GTPase RhoA. Taken together, these results demonstrate that mouse neutrophils express functional Tas2R126/143 and suggest a role for Tas2R126/143–ROCK–MLC2-dependent signaling in the regulation of neutrophil migration.
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spelling pubmed-94363162022-09-02 Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway Kobayashi, Daichi Watarai, Tomoya Ozawa, Madoka Kanda, Yasuhiro Saika, Fumihiro Kiguchi, Norikazu Takeuchi, Arata Ikawa, Masahito Matsuzaki, Shinsuke Katakai, Tomoya Front Immunol Immunology Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled receptors that are expressed in the oral cavity and serve to detect substances with bitter tastes in foods and medicines. Recent evidence suggests that Tas2Rs are also expressed extraorally, including in immune cells. However, the role of Tas2Rs in immune cells remains controversial. Here, we demonstrate that Tas2R126, Tas2R135, and Tas2R143 are expressed in mouse neutrophils, but not in other immune cells such as macrophages or T and B lymphocytes. Treatment of bone marrow-derived neutrophils from wild-type mice with the Tas2R126/143 agonists arbutin and d-salicin led to enhanced C-X-C motif chemokine ligand 2 (CXCL2)-stimulated migration in vitro, but this response was not observed in neutrophils from Tas2r126/135/143-deficient mice. Enhancement of CXCL2-stimulated migration by Tas2R agonists was accompanied by increased phosphorylation of myosin light chain 2 (MLC2) and was blocked by pretreatment of neutrophils with inhibitors of Rho-associated coiled-coil-containing protein kinase (ROCK), but not by inhibitors of the small GTPase RhoA. Taken together, these results demonstrate that mouse neutrophils express functional Tas2R126/143 and suggest a role for Tas2R126/143–ROCK–MLC2-dependent signaling in the regulation of neutrophil migration. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9436316/ /pubmed/36059440 http://dx.doi.org/10.3389/fimmu.2022.973880 Text en Copyright © 2022 Kobayashi, Watarai, Ozawa, Kanda, Saika, Kiguchi, Takeuchi, Ikawa, Matsuzaki and Katakai https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Kobayashi, Daichi
Watarai, Tomoya
Ozawa, Madoka
Kanda, Yasuhiro
Saika, Fumihiro
Kiguchi, Norikazu
Takeuchi, Arata
Ikawa, Masahito
Matsuzaki, Shinsuke
Katakai, Tomoya
Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title_full Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title_fullStr Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title_full_unstemmed Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title_short Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
title_sort tas2r signaling enhances mouse neutrophil migration via a rock-dependent pathway
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436316/
https://www.ncbi.nlm.nih.gov/pubmed/36059440
http://dx.doi.org/10.3389/fimmu.2022.973880
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