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Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis

Neutrophils have been recognized to play an important role in the pathogenesis of tuberculosis in recent years. Interferon-induced blood transcriptional signatures in ATB are predominantly driven by neutrophils. In this study, we performed global RNA-seq on peripheral blood neutrophils from active t...

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Autores principales: Geng, Xingzhu, Wu, Xiaolin, Yang, Qianting, Xin, Henan, Zhang, Bin, Wang, Dakuan, Liu, Liguo, Liu, Song, Chen, Qi, Liu, Zisen, Zhang, Mingxia, Pan, Shouguo, Zhang, Xiaobing, Gao, Lei, Jin, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436479/
https://www.ncbi.nlm.nih.gov/pubmed/36059536
http://dx.doi.org/10.3389/fimmu.2022.954221
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author Geng, Xingzhu
Wu, Xiaolin
Yang, Qianting
Xin, Henan
Zhang, Bin
Wang, Dakuan
Liu, Liguo
Liu, Song
Chen, Qi
Liu, Zisen
Zhang, Mingxia
Pan, Shouguo
Zhang, Xiaobing
Gao, Lei
Jin, Qi
author_facet Geng, Xingzhu
Wu, Xiaolin
Yang, Qianting
Xin, Henan
Zhang, Bin
Wang, Dakuan
Liu, Liguo
Liu, Song
Chen, Qi
Liu, Zisen
Zhang, Mingxia
Pan, Shouguo
Zhang, Xiaobing
Gao, Lei
Jin, Qi
author_sort Geng, Xingzhu
collection PubMed
description Neutrophils have been recognized to play an important role in the pathogenesis of tuberculosis in recent years. Interferon-induced blood transcriptional signatures in ATB are predominantly driven by neutrophils. In this study, we performed global RNA-seq on peripheral blood neutrophils from active tuberculosis patients (ATB, n=15); latent tuberculosis infections (LTBI, n=22); and healthy controls (HC, n=21). The results showed that greater perturbations of gene expression patterns happened in neutrophils from ATB individuals than HC or those with LTBI, and a total of 344 differentially expressed genes (DEGs) were observed. Functional enrichment analysis showed that besides the interferon signaling pathway, multiple pattern recognition receptor pathways were significantly activated in ATB, such as NOD-like receptors and Toll-like receptors. Meanwhile, we also observed that the expression of genes related to endocytosis, secretory granules, and neutrophils degranulation were downregulated. Our data also showed that the NF-κB signaling pathway might be inhibited in patients with ATB, which could increase Mycobacterium tuberculosis survival and lead to active tuberculosis status. Furthermore, we validated the accuracy of some differentially expressed genes in an independent cohort using quantitative PCR, and obtained three novel genes (RBM3, CSRNP1, SRSF5) with the ability to discriminate active tuberculosis from LTBI and HC.
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spelling pubmed-94364792022-09-02 Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis Geng, Xingzhu Wu, Xiaolin Yang, Qianting Xin, Henan Zhang, Bin Wang, Dakuan Liu, Liguo Liu, Song Chen, Qi Liu, Zisen Zhang, Mingxia Pan, Shouguo Zhang, Xiaobing Gao, Lei Jin, Qi Front Immunol Immunology Neutrophils have been recognized to play an important role in the pathogenesis of tuberculosis in recent years. Interferon-induced blood transcriptional signatures in ATB are predominantly driven by neutrophils. In this study, we performed global RNA-seq on peripheral blood neutrophils from active tuberculosis patients (ATB, n=15); latent tuberculosis infections (LTBI, n=22); and healthy controls (HC, n=21). The results showed that greater perturbations of gene expression patterns happened in neutrophils from ATB individuals than HC or those with LTBI, and a total of 344 differentially expressed genes (DEGs) were observed. Functional enrichment analysis showed that besides the interferon signaling pathway, multiple pattern recognition receptor pathways were significantly activated in ATB, such as NOD-like receptors and Toll-like receptors. Meanwhile, we also observed that the expression of genes related to endocytosis, secretory granules, and neutrophils degranulation were downregulated. Our data also showed that the NF-κB signaling pathway might be inhibited in patients with ATB, which could increase Mycobacterium tuberculosis survival and lead to active tuberculosis status. Furthermore, we validated the accuracy of some differentially expressed genes in an independent cohort using quantitative PCR, and obtained three novel genes (RBM3, CSRNP1, SRSF5) with the ability to discriminate active tuberculosis from LTBI and HC. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9436479/ /pubmed/36059536 http://dx.doi.org/10.3389/fimmu.2022.954221 Text en Copyright © 2022 Geng, Wu, Yang, Xin, Zhang, Wang, Liu, Liu, Chen, Liu, Zhang, Pan, Zhang, Gao and Jin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Geng, Xingzhu
Wu, Xiaolin
Yang, Qianting
Xin, Henan
Zhang, Bin
Wang, Dakuan
Liu, Liguo
Liu, Song
Chen, Qi
Liu, Zisen
Zhang, Mingxia
Pan, Shouguo
Zhang, Xiaobing
Gao, Lei
Jin, Qi
Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title_full Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title_fullStr Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title_full_unstemmed Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title_short Whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
title_sort whole transcriptome sequencing reveals neutrophils’ transcriptional landscape associated with active tuberculosis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436479/
https://www.ncbi.nlm.nih.gov/pubmed/36059536
http://dx.doi.org/10.3389/fimmu.2022.954221
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