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Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker

Rice blast is a detrimental rice disease caused by the fungus Magnaporthe oryzae. Here, we identified a resistance gene from the rice cultivar Fuhui 2663 which is resistant to the rice blast isolate KJ201. Through isolated population analyses and sequencing approaches, the candidate gene was traced...

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Autores principales: He, Niqing, Huang, Fenghuang, Yu, Mingxiang, Zhu, Yebao, Li, Qingshun Q., Yang, Dewei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437026/
https://www.ncbi.nlm.nih.gov/pubmed/36050368
http://dx.doi.org/10.1038/s41598-022-19004-y
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author He, Niqing
Huang, Fenghuang
Yu, Mingxiang
Zhu, Yebao
Li, Qingshun Q.
Yang, Dewei
author_facet He, Niqing
Huang, Fenghuang
Yu, Mingxiang
Zhu, Yebao
Li, Qingshun Q.
Yang, Dewei
author_sort He, Niqing
collection PubMed
description Rice blast is a detrimental rice disease caused by the fungus Magnaporthe oryzae. Here, we identified a resistance gene from the rice cultivar Fuhui 2663 which is resistant to the rice blast isolate KJ201. Through isolated population analyses and sequencing approaches, the candidate gene was traced to chromosome 12. With the use of a map-based cloning strategy, the resistance gene was ultimately mapped to an 80-kb resistance locus region containing the Pita gene. Candidate gene prediction and cDNA sequencing indicated that the target resistance gene in Fuhui 2663 was allelic to Pita, thus being referred to as Pita-Fuhui2663 hereafter. Further analysis showed that the Fuhui 2663 protein had one amino acid change: Ala (A) residue 918 in Pita-Fuhui2663 was replaced by Ser (S) in Pita-S, leading to a significant change in the 3D structure of the Pita-S protein. CRISPR/Cas9 knockout experiments confirmed that Pita-Fuhui2663 is responsible for the resistance phenotype of Fuhui 2663. Importantly, Pita-Fuhui2663 did not affect the main agronomic traits of the variety compared to the Pita gene as verified by knockout experiments, indicative of potential applications of Pita-Fuhui2663 in broader breeding programs. Furthermore, a Pita-Fuhui2663-dCAPS molecular marker with good specificity and high efficiency was developed to facilitate rice breeding for resistance to this devastating disease.
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spelling pubmed-94370262022-09-03 Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker He, Niqing Huang, Fenghuang Yu, Mingxiang Zhu, Yebao Li, Qingshun Q. Yang, Dewei Sci Rep Article Rice blast is a detrimental rice disease caused by the fungus Magnaporthe oryzae. Here, we identified a resistance gene from the rice cultivar Fuhui 2663 which is resistant to the rice blast isolate KJ201. Through isolated population analyses and sequencing approaches, the candidate gene was traced to chromosome 12. With the use of a map-based cloning strategy, the resistance gene was ultimately mapped to an 80-kb resistance locus region containing the Pita gene. Candidate gene prediction and cDNA sequencing indicated that the target resistance gene in Fuhui 2663 was allelic to Pita, thus being referred to as Pita-Fuhui2663 hereafter. Further analysis showed that the Fuhui 2663 protein had one amino acid change: Ala (A) residue 918 in Pita-Fuhui2663 was replaced by Ser (S) in Pita-S, leading to a significant change in the 3D structure of the Pita-S protein. CRISPR/Cas9 knockout experiments confirmed that Pita-Fuhui2663 is responsible for the resistance phenotype of Fuhui 2663. Importantly, Pita-Fuhui2663 did not affect the main agronomic traits of the variety compared to the Pita gene as verified by knockout experiments, indicative of potential applications of Pita-Fuhui2663 in broader breeding programs. Furthermore, a Pita-Fuhui2663-dCAPS molecular marker with good specificity and high efficiency was developed to facilitate rice breeding for resistance to this devastating disease. Nature Publishing Group UK 2022-09-01 /pmc/articles/PMC9437026/ /pubmed/36050368 http://dx.doi.org/10.1038/s41598-022-19004-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
He, Niqing
Huang, Fenghuang
Yu, Mingxiang
Zhu, Yebao
Li, Qingshun Q.
Yang, Dewei
Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title_full Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title_fullStr Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title_full_unstemmed Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title_short Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
title_sort analysis of a rice blast resistance gene pita-fuhui2663 and development of selection marker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437026/
https://www.ncbi.nlm.nih.gov/pubmed/36050368
http://dx.doi.org/10.1038/s41598-022-19004-y
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