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Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries

While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull fractures. To...

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Autores principales: Dill, Larissa K., Sims, Natalie A., Shad, Ali, Anyaegbu, Chidozie, Warnock, Andrew, Mao, Yilin, Fitzgerald, Melinda, Semple, Bridgette D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437056/
https://www.ncbi.nlm.nih.gov/pubmed/36050485
http://dx.doi.org/10.1038/s41598-022-18643-5
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author Dill, Larissa K.
Sims, Natalie A.
Shad, Ali
Anyaegbu, Chidozie
Warnock, Andrew
Mao, Yilin
Fitzgerald, Melinda
Semple, Bridgette D.
author_facet Dill, Larissa K.
Sims, Natalie A.
Shad, Ali
Anyaegbu, Chidozie
Warnock, Andrew
Mao, Yilin
Fitzgerald, Melinda
Semple, Bridgette D.
author_sort Dill, Larissa K.
collection PubMed
description While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull fractures. To address this, young adult female Piebald Viral Glaxo rats received sham, 1×, 2× or 3× closed-head mTBIs delivered at 24 h intervals, using a weight-drop device custom-built for reproducible impact. Skull bones were collected at 2 or 10 weeks after the final injury/sham procedure, imaged by micro computed tomography and analyzed at predetermined regions of interest. In the interparietal bone, proximal to the injury site, modest increases in bone thickness were observed at 2 weeks, particularly following 2× and 3× mTBI. By 10 weeks, 2× mTBI induced a robust increase in the volume and thickness of the interparietal bone, alongside a corresponding decrease in the volume of marrow cavities in the diploë region. In contrast, neither parietal nor frontal skull samples were affected by rmTBI. Our findings demonstrate time- and location-dependent effects of rmTBI on cranial bone structure, highlighting a need to consider microstructural alterations to cranial bone when assessing the consequences of rmTBI.
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spelling pubmed-94370562022-09-03 Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries Dill, Larissa K. Sims, Natalie A. Shad, Ali Anyaegbu, Chidozie Warnock, Andrew Mao, Yilin Fitzgerald, Melinda Semple, Bridgette D. Sci Rep Article While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull fractures. To address this, young adult female Piebald Viral Glaxo rats received sham, 1×, 2× or 3× closed-head mTBIs delivered at 24 h intervals, using a weight-drop device custom-built for reproducible impact. Skull bones were collected at 2 or 10 weeks after the final injury/sham procedure, imaged by micro computed tomography and analyzed at predetermined regions of interest. In the interparietal bone, proximal to the injury site, modest increases in bone thickness were observed at 2 weeks, particularly following 2× and 3× mTBI. By 10 weeks, 2× mTBI induced a robust increase in the volume and thickness of the interparietal bone, alongside a corresponding decrease in the volume of marrow cavities in the diploë region. In contrast, neither parietal nor frontal skull samples were affected by rmTBI. Our findings demonstrate time- and location-dependent effects of rmTBI on cranial bone structure, highlighting a need to consider microstructural alterations to cranial bone when assessing the consequences of rmTBI. Nature Publishing Group UK 2022-09-01 /pmc/articles/PMC9437056/ /pubmed/36050485 http://dx.doi.org/10.1038/s41598-022-18643-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dill, Larissa K.
Sims, Natalie A.
Shad, Ali
Anyaegbu, Chidozie
Warnock, Andrew
Mao, Yilin
Fitzgerald, Melinda
Semple, Bridgette D.
Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title_full Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title_fullStr Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title_full_unstemmed Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title_short Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
title_sort localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437056/
https://www.ncbi.nlm.nih.gov/pubmed/36050485
http://dx.doi.org/10.1038/s41598-022-18643-5
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