Cargando…
Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing
Atrial natriuretic peptide (ANP) plays a pivotal role in the regulation of the cardiovascular system. The ANP level increases during atrial fibrillation (AF), suggesting that AF may provoke ANP secretion, but its potential mechanism is still unclear. In the present study, the potential mechanisms of...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437264/ https://www.ncbi.nlm.nih.gov/pubmed/36060704 http://dx.doi.org/10.3389/fphys.2022.861981 |
_version_ | 1784781554849939456 |
---|---|
author | Cao, Shuxia Han, Chengyong Xuan, Chunhua Li, Xiangdan Wen, Jing Xu, Dongyuan |
author_facet | Cao, Shuxia Han, Chengyong Xuan, Chunhua Li, Xiangdan Wen, Jing Xu, Dongyuan |
author_sort | Cao, Shuxia |
collection | PubMed |
description | Atrial natriuretic peptide (ANP) plays a pivotal role in the regulation of the cardiovascular system. The ANP level increases during atrial fibrillation (AF), suggesting that AF may provoke ANP secretion, but its potential mechanism is still unclear. In the present study, the potential mechanisms of rapid atrial pacing (RAP) regulating ANP secretion was explored. Rabbits were subjected to burst RAP, ANP secretion increased whereas cyclic guanosine monophosphate (cGMP) concentrations decreased during RAP. The p-Akt and p-GSK-3β levels decreased in atrial tissues. Natriuretic peptide receptor A (NPR-A) protein and particulate guanylate cyclase (PGC) activity were detected. The sensitivity of NPR-A to ANP decreased, leading to the decrease of PGC activity. Also, the isolated atrial perfusion system were made in the rabbit model, cGMP was shown to inhibit ANP secretion, and the Akt inhibitor LY294002 (LY) and GSK-3β inhibitor SB216763 (SB) attenuated the inhibitory effects of cGMP on ANP secretion and enhanced the inhibitory effects of cGMP on atrial dynamics. In conclusion, NPR-A interacts with ANP to regulate PGC expression, and influence the expression of cGMP during RAP, which involves in the Akt/GSK-3β signaling pathway. From the aforementioned points we conclude that cGMP regulates ANP secretion by the Akt/GSK-3β signaling pathway during atrial pacing. |
format | Online Article Text |
id | pubmed-9437264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94372642022-09-03 Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing Cao, Shuxia Han, Chengyong Xuan, Chunhua Li, Xiangdan Wen, Jing Xu, Dongyuan Front Physiol Physiology Atrial natriuretic peptide (ANP) plays a pivotal role in the regulation of the cardiovascular system. The ANP level increases during atrial fibrillation (AF), suggesting that AF may provoke ANP secretion, but its potential mechanism is still unclear. In the present study, the potential mechanisms of rapid atrial pacing (RAP) regulating ANP secretion was explored. Rabbits were subjected to burst RAP, ANP secretion increased whereas cyclic guanosine monophosphate (cGMP) concentrations decreased during RAP. The p-Akt and p-GSK-3β levels decreased in atrial tissues. Natriuretic peptide receptor A (NPR-A) protein and particulate guanylate cyclase (PGC) activity were detected. The sensitivity of NPR-A to ANP decreased, leading to the decrease of PGC activity. Also, the isolated atrial perfusion system were made in the rabbit model, cGMP was shown to inhibit ANP secretion, and the Akt inhibitor LY294002 (LY) and GSK-3β inhibitor SB216763 (SB) attenuated the inhibitory effects of cGMP on ANP secretion and enhanced the inhibitory effects of cGMP on atrial dynamics. In conclusion, NPR-A interacts with ANP to regulate PGC expression, and influence the expression of cGMP during RAP, which involves in the Akt/GSK-3β signaling pathway. From the aforementioned points we conclude that cGMP regulates ANP secretion by the Akt/GSK-3β signaling pathway during atrial pacing. Frontiers Media S.A. 2022-08-19 /pmc/articles/PMC9437264/ /pubmed/36060704 http://dx.doi.org/10.3389/fphys.2022.861981 Text en Copyright © 2022 Cao, Han, Xuan, Li, Wen and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Cao, Shuxia Han, Chengyong Xuan, Chunhua Li, Xiangdan Wen, Jing Xu, Dongyuan Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title | Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title_full | Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title_fullStr | Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title_full_unstemmed | Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title_short | Effects of cGMP/Akt/GSK-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
title_sort | effects of cgmp/akt/gsk-3β signaling pathway on atrial natriuretic peptide secretion in rabbits with rapid atrial pacing |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437264/ https://www.ncbi.nlm.nih.gov/pubmed/36060704 http://dx.doi.org/10.3389/fphys.2022.861981 |
work_keys_str_mv | AT caoshuxia effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing AT hanchengyong effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing AT xuanchunhua effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing AT lixiangdan effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing AT wenjing effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing AT xudongyuan effectsofcgmpaktgsk3bsignalingpathwayonatrialnatriureticpeptidesecretioninrabbitswithrapidatrialpacing |