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Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China
Apis mellifera filamentous virus (AmFV) is a large DNA virus that is endemic in honeybee colonies. The genome sequence of the AmFV Swiss isolate (AmFV CH–C05) has been reported, but so far very few molecular studies have been conducted on this virus. In this study, we isolated and purified AmFV (AmF...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Wuhan Institute of Virology, Chinese Academy of Sciences
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437511/ https://www.ncbi.nlm.nih.gov/pubmed/35527222 http://dx.doi.org/10.1016/j.virs.2022.02.007 |
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author | Yang, Dahe Wang, Jun Wang, Xi Deng, Fei Diao, Qingyun Wang, Manli Hu, Zhihong Hou, Chunsheng |
author_facet | Yang, Dahe Wang, Jun Wang, Xi Deng, Fei Diao, Qingyun Wang, Manli Hu, Zhihong Hou, Chunsheng |
author_sort | Yang, Dahe |
collection | PubMed |
description | Apis mellifera filamentous virus (AmFV) is a large DNA virus that is endemic in honeybee colonies. The genome sequence of the AmFV Swiss isolate (AmFV CH–C05) has been reported, but so far very few molecular studies have been conducted on this virus. In this study, we isolated and purified AmFV (AmFV CN) from Chinese honeybee (Apis mellifera) colonies and elucidated its genomics and proteomics. Electron microscopy showed ovoid purified virions with dimensions of 300–500 × 210–285 nm, wrapping a 3165 × 40 nm filamentous nucleocapsid in three figure-eight loops. Unlike AmFV CH–C05, which was reported to have a circular genome, our data suggest that AmFV CN has a linear genome of approximately 493 kb. A total of 197 ORFs were identified, among which 36 putative genes including 18 baculoviral homologs were annotated. The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%. Several ORFs were newly annotated in AmFV CN, including homologs of per os infectivity factor 4 (PIF4) and a putative integrase. Phylogenomic analysis placed AmFVs on a separate branch within the newly proposed virus class Naldaviricetes. Proteomic analysis revealed 47 AmFV virion-associated proteins, of which 14 had over 50% sequence coverage, suggesting that they are likely to be main structural proteins. In addition, all six of the annotated PIFs (PIF-0–5) were identified by proteomics, suggesting that they may function as entry factors in AmFV infection. This study provides fundamental information regarding the molecular biology of AmFV. |
format | Online Article Text |
id | pubmed-9437511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wuhan Institute of Virology, Chinese Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-94375112022-09-09 Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China Yang, Dahe Wang, Jun Wang, Xi Deng, Fei Diao, Qingyun Wang, Manli Hu, Zhihong Hou, Chunsheng Virol Sin Research Article Apis mellifera filamentous virus (AmFV) is a large DNA virus that is endemic in honeybee colonies. The genome sequence of the AmFV Swiss isolate (AmFV CH–C05) has been reported, but so far very few molecular studies have been conducted on this virus. In this study, we isolated and purified AmFV (AmFV CN) from Chinese honeybee (Apis mellifera) colonies and elucidated its genomics and proteomics. Electron microscopy showed ovoid purified virions with dimensions of 300–500 × 210–285 nm, wrapping a 3165 × 40 nm filamentous nucleocapsid in three figure-eight loops. Unlike AmFV CH–C05, which was reported to have a circular genome, our data suggest that AmFV CN has a linear genome of approximately 493 kb. A total of 197 ORFs were identified, among which 36 putative genes including 18 baculoviral homologs were annotated. The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%. Several ORFs were newly annotated in AmFV CN, including homologs of per os infectivity factor 4 (PIF4) and a putative integrase. Phylogenomic analysis placed AmFVs on a separate branch within the newly proposed virus class Naldaviricetes. Proteomic analysis revealed 47 AmFV virion-associated proteins, of which 14 had over 50% sequence coverage, suggesting that they are likely to be main structural proteins. In addition, all six of the annotated PIFs (PIF-0–5) were identified by proteomics, suggesting that they may function as entry factors in AmFV infection. This study provides fundamental information regarding the molecular biology of AmFV. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-02-25 /pmc/articles/PMC9437511/ /pubmed/35527222 http://dx.doi.org/10.1016/j.virs.2022.02.007 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Yang, Dahe Wang, Jun Wang, Xi Deng, Fei Diao, Qingyun Wang, Manli Hu, Zhihong Hou, Chunsheng Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title_full | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title_fullStr | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title_full_unstemmed | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title_short | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China |
title_sort | genomics and proteomics of apis mellifera filamentous virus isolated from honeybees in china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437511/ https://www.ncbi.nlm.nih.gov/pubmed/35527222 http://dx.doi.org/10.1016/j.virs.2022.02.007 |
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