Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

• Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase. • IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1. • IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vi...

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Detalles Bibliográficos
Autores principales: Chen, Yuanzhi, Shen, Chenguang, Chen, Jing, Chen, Junyu, Chen, Fentian, Zhang, Limin, Liu, Xue, Chen, Siyuan, Xue, Sen, Liu, Yongliang, Tang, Jixian, Yuan, Quan, Chen, Yixin, Luo, Wenxin, Xia, Ningshao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437528/
https://www.ncbi.nlm.nih.gov/pubmed/35331970
http://dx.doi.org/10.1016/j.virs.2022.03.009
Descripción
Sumario:• Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase. • IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1. • IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

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