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Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells
• Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase. • IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1. • IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vi...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Wuhan Institute of Virology, Chinese Academy of Sciences
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437528/ https://www.ncbi.nlm.nih.gov/pubmed/35331970 http://dx.doi.org/10.1016/j.virs.2022.03.009 |
| _version_ | 1784781635301933056 |
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| author | Chen, Yuanzhi Shen, Chenguang Chen, Jing Chen, Junyu Chen, Fentian Zhang, Limin Liu, Xue Chen, Siyuan Xue, Sen Liu, Yongliang Tang, Jixian Yuan, Quan Chen, Yixin Luo, Wenxin Xia, Ningshao |
| author_facet | Chen, Yuanzhi Shen, Chenguang Chen, Jing Chen, Junyu Chen, Fentian Zhang, Limin Liu, Xue Chen, Siyuan Xue, Sen Liu, Yongliang Tang, Jixian Yuan, Quan Chen, Yixin Luo, Wenxin Xia, Ningshao |
| author_sort | Chen, Yuanzhi |
| collection | PubMed |
| description | • Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase. • IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1. • IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo. |
| format | Online Article Text |
| id | pubmed-9437528 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Wuhan Institute of Virology, Chinese Academy of Sciences |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-94375282022-09-09 Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells Chen, Yuanzhi Shen, Chenguang Chen, Jing Chen, Junyu Chen, Fentian Zhang, Limin Liu, Xue Chen, Siyuan Xue, Sen Liu, Yongliang Tang, Jixian Yuan, Quan Chen, Yixin Luo, Wenxin Xia, Ningshao Virol Sin Letter • Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase. • IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1. • IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-03-22 /pmc/articles/PMC9437528/ /pubmed/35331970 http://dx.doi.org/10.1016/j.virs.2022.03.009 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Letter Chen, Yuanzhi Shen, Chenguang Chen, Jing Chen, Junyu Chen, Fentian Zhang, Limin Liu, Xue Chen, Siyuan Xue, Sen Liu, Yongliang Tang, Jixian Yuan, Quan Chen, Yixin Luo, Wenxin Xia, Ningshao Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title | Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title_full | Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title_fullStr | Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title_full_unstemmed | Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title_short | Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells |
| title_sort | development of functional antibodies against influenza b virus by activation-induced cytidine deaminase in hybridoma cells |
| topic | Letter |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437528/ https://www.ncbi.nlm.nih.gov/pubmed/35331970 http://dx.doi.org/10.1016/j.virs.2022.03.009 |
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