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Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption
One of the greatest threats to wild strawberries (Fragaria vesca Mara des Bois) after harvest is the highly perishability at ambient temperature. Breeders have successfully met the quality demands of consumers, but the prevention of waste after harvest in fleshy fruits is still pending. Most of the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437593/ https://www.ncbi.nlm.nih.gov/pubmed/36061763 http://dx.doi.org/10.3389/fpls.2022.983976 |
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author | del Olmo, Ivan Romero, Irene Alvarez, Maria Dolores Tarradas, Rosa Sanchez-Ballesta, Maria Teresa Escribano, Maria Isabel Merodio, Carmen |
author_facet | del Olmo, Ivan Romero, Irene Alvarez, Maria Dolores Tarradas, Rosa Sanchez-Ballesta, Maria Teresa Escribano, Maria Isabel Merodio, Carmen |
author_sort | del Olmo, Ivan |
collection | PubMed |
description | One of the greatest threats to wild strawberries (Fragaria vesca Mara des Bois) after harvest is the highly perishability at ambient temperature. Breeders have successfully met the quality demands of consumers, but the prevention of waste after harvest in fleshy fruits is still pending. Most of the waste is due to the accelerated progress of senescence-like process after harvest linked to a rapid loss of water and firmness at ambient temperature. The storage life of strawberries increases at low temperature, but their quality is limited by the loss of cell structure. The application of high CO(2) concentrations increased firmness during cold storage. However, the key genes related to resistance to softening and cell wall disassembly following transference from cold storage at 20°C remain unclear. Therefore, we performed RNA-seq analysis, constructing a weighted gene co-expression network analysis (WGCNA) to identify which molecular determinants play a role in cell wall integrity, using strawberries with contrasting storage conditions, CO(2)-cold stored (CCS), air-cold stored (ACS), non-cold stored (NCS) kept at ambient temperature, and intact fruit at harvest (AH). The hub genes associated with the cell wall structural architecture of firmer CO(2)-treated strawberries revealed xyloglucans stabilization attributed mainly to a down-regulation of Csl E1, XTH 15, Exp-like B1 and the maintenance of expression levels of nucleotide sugars transferases such as GMP and FUT as well as improved lamella integrity linked to a down-regulation of RG-lyase, PL-like and PME. The preservation of cell wall elasticity together with the up-regulation of LEA, EXPA4, and MATE, required to maintain cell turgor, is the mechanisms controlled by high CO(2). In stressed air-cold stored strawberries, in addition to an acute softening, there is a preferential transcript accumulation of genes involved in lignin and raffinose pathways. Non-cold stored strawberries kept at 20°C after harvest are characterized by an enrichment in genes mainly involved in oxidative stress and up-expression of genes involved in jasmonate biosynthesis. The present results on transcriptomic analysis of CO(2)-treated strawberries with enhanced resistance to softening and oxidative stress at consumption will help to improve breeding strategies of both wild and cultivated strawberries. |
format | Online Article Text |
id | pubmed-9437593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94375932022-09-03 Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption del Olmo, Ivan Romero, Irene Alvarez, Maria Dolores Tarradas, Rosa Sanchez-Ballesta, Maria Teresa Escribano, Maria Isabel Merodio, Carmen Front Plant Sci Plant Science One of the greatest threats to wild strawberries (Fragaria vesca Mara des Bois) after harvest is the highly perishability at ambient temperature. Breeders have successfully met the quality demands of consumers, but the prevention of waste after harvest in fleshy fruits is still pending. Most of the waste is due to the accelerated progress of senescence-like process after harvest linked to a rapid loss of water and firmness at ambient temperature. The storage life of strawberries increases at low temperature, but their quality is limited by the loss of cell structure. The application of high CO(2) concentrations increased firmness during cold storage. However, the key genes related to resistance to softening and cell wall disassembly following transference from cold storage at 20°C remain unclear. Therefore, we performed RNA-seq analysis, constructing a weighted gene co-expression network analysis (WGCNA) to identify which molecular determinants play a role in cell wall integrity, using strawberries with contrasting storage conditions, CO(2)-cold stored (CCS), air-cold stored (ACS), non-cold stored (NCS) kept at ambient temperature, and intact fruit at harvest (AH). The hub genes associated with the cell wall structural architecture of firmer CO(2)-treated strawberries revealed xyloglucans stabilization attributed mainly to a down-regulation of Csl E1, XTH 15, Exp-like B1 and the maintenance of expression levels of nucleotide sugars transferases such as GMP and FUT as well as improved lamella integrity linked to a down-regulation of RG-lyase, PL-like and PME. The preservation of cell wall elasticity together with the up-regulation of LEA, EXPA4, and MATE, required to maintain cell turgor, is the mechanisms controlled by high CO(2). In stressed air-cold stored strawberries, in addition to an acute softening, there is a preferential transcript accumulation of genes involved in lignin and raffinose pathways. Non-cold stored strawberries kept at 20°C after harvest are characterized by an enrichment in genes mainly involved in oxidative stress and up-expression of genes involved in jasmonate biosynthesis. The present results on transcriptomic analysis of CO(2)-treated strawberries with enhanced resistance to softening and oxidative stress at consumption will help to improve breeding strategies of both wild and cultivated strawberries. Frontiers Media S.A. 2022-08-19 /pmc/articles/PMC9437593/ /pubmed/36061763 http://dx.doi.org/10.3389/fpls.2022.983976 Text en Copyright © 2022 del Olmo, Romero, Alvarez, Tarradas, Sanchez-Ballesta, Escribano and Merodio. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science del Olmo, Ivan Romero, Irene Alvarez, Maria Dolores Tarradas, Rosa Sanchez-Ballesta, Maria Teresa Escribano, Maria Isabel Merodio, Carmen Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title | Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title_full | Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title_fullStr | Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title_full_unstemmed | Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title_short | Transcriptomic analysis of CO(2)-treated strawberries (Fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
title_sort | transcriptomic analysis of co(2)-treated strawberries (fragaria vesca) with enhanced resistance to softening and oxidative stress at consumption |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437593/ https://www.ncbi.nlm.nih.gov/pubmed/36061763 http://dx.doi.org/10.3389/fpls.2022.983976 |
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