Cargando…
A microfluidic cell chip for virus isolation via rapid screening for permissive cells
Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wuhan Institute of Virology, Chinese Academy of Sciences
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437619/ https://www.ncbi.nlm.nih.gov/pubmed/35504535 http://dx.doi.org/10.1016/j.virs.2022.04.011 |
_version_ | 1784781659084685312 |
---|---|
author | Su, Weide Qiu, Jingjiang Mei, Ying Zhang, Xian-En He, Yong Li, Feng |
author_facet | Su, Weide Qiu, Jingjiang Mei, Ying Zhang, Xian-En He, Yong Li, Feng |
author_sort | Su, Weide |
collection | PubMed |
description | Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way. |
format | Online Article Text |
id | pubmed-9437619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wuhan Institute of Virology, Chinese Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-94376192022-09-09 A microfluidic cell chip for virus isolation via rapid screening for permissive cells Su, Weide Qiu, Jingjiang Mei, Ying Zhang, Xian-En He, Yong Li, Feng Virol Sin Research Article Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-05-02 /pmc/articles/PMC9437619/ /pubmed/35504535 http://dx.doi.org/10.1016/j.virs.2022.04.011 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Su, Weide Qiu, Jingjiang Mei, Ying Zhang, Xian-En He, Yong Li, Feng A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title | A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title_full | A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title_fullStr | A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title_full_unstemmed | A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title_short | A microfluidic cell chip for virus isolation via rapid screening for permissive cells |
title_sort | microfluidic cell chip for virus isolation via rapid screening for permissive cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437619/ https://www.ncbi.nlm.nih.gov/pubmed/35504535 http://dx.doi.org/10.1016/j.virs.2022.04.011 |
work_keys_str_mv | AT suweide amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT qiujingjiang amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT meiying amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT zhangxianen amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT heyong amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT lifeng amicrofluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT suweide microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT qiujingjiang microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT meiying microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT zhangxianen microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT heyong microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells AT lifeng microfluidiccellchipforvirusisolationviarapidscreeningforpermissivecells |