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A microfluidic cell chip for virus isolation via rapid screening for permissive cells

Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permi...

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Autores principales: Su, Weide, Qiu, Jingjiang, Mei, Ying, Zhang, Xian-En, He, Yong, Li, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437619/
https://www.ncbi.nlm.nih.gov/pubmed/35504535
http://dx.doi.org/10.1016/j.virs.2022.04.011
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author Su, Weide
Qiu, Jingjiang
Mei, Ying
Zhang, Xian-En
He, Yong
Li, Feng
author_facet Su, Weide
Qiu, Jingjiang
Mei, Ying
Zhang, Xian-En
He, Yong
Li, Feng
author_sort Su, Weide
collection PubMed
description Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way.
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spelling pubmed-94376192022-09-09 A microfluidic cell chip for virus isolation via rapid screening for permissive cells Su, Weide Qiu, Jingjiang Mei, Ying Zhang, Xian-En He, Yong Li, Feng Virol Sin Research Article Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-05-02 /pmc/articles/PMC9437619/ /pubmed/35504535 http://dx.doi.org/10.1016/j.virs.2022.04.011 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Su, Weide
Qiu, Jingjiang
Mei, Ying
Zhang, Xian-En
He, Yong
Li, Feng
A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title_full A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title_fullStr A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title_full_unstemmed A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title_short A microfluidic cell chip for virus isolation via rapid screening for permissive cells
title_sort microfluidic cell chip for virus isolation via rapid screening for permissive cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437619/
https://www.ncbi.nlm.nih.gov/pubmed/35504535
http://dx.doi.org/10.1016/j.virs.2022.04.011
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