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Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)

Ethnopharmacological relevance: Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities. Aim of the Study: The present...

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Autores principales: Nicolescu, Alexandru, Babotă, Mihai, Ilea, Maria, Dias, Maria Inês, Calhelha, Ricardo C., Gavrilaș, Laura, Rocchetti, Gabriele, Crișan, Gianina, Mocan, Andrei, Barros, Lillian, Pârvu, Alina Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437640/
https://www.ncbi.nlm.nih.gov/pubmed/36059937
http://dx.doi.org/10.3389/fphar.2022.975800
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author Nicolescu, Alexandru
Babotă, Mihai
Ilea, Maria
Dias, Maria Inês
Calhelha, Ricardo C.
Gavrilaș, Laura
Rocchetti, Gabriele
Crișan, Gianina
Mocan, Andrei
Barros, Lillian
Pârvu, Alina Elena
author_facet Nicolescu, Alexandru
Babotă, Mihai
Ilea, Maria
Dias, Maria Inês
Calhelha, Ricardo C.
Gavrilaș, Laura
Rocchetti, Gabriele
Crișan, Gianina
Mocan, Andrei
Barros, Lillian
Pârvu, Alina Elena
author_sort Nicolescu, Alexandru
collection PubMed
description Ethnopharmacological relevance: Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities. Aim of the Study: The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of S. glutinosa extracts, as well as the in vivo anti-inflammatory activity. Materials and Methods: Infusions and 70% (v:v) ethanol solution extracts of S. glutinosa stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MS(n) method, and total phenolic and flavonoid content, as well as in vitro antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of in vivo effects of the extracts, using diclofenac as an anti-inflammatory control. Results: The highest inhibitory α-glucosidase activity was determined to be IC(50) = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI(50) = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. In vivo administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production. Conclusion: Our findings indicate that S. glutinosa extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By in vivo administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from S. glutinosa.
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spelling pubmed-94376402022-09-03 Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) Nicolescu, Alexandru Babotă, Mihai Ilea, Maria Dias, Maria Inês Calhelha, Ricardo C. Gavrilaș, Laura Rocchetti, Gabriele Crișan, Gianina Mocan, Andrei Barros, Lillian Pârvu, Alina Elena Front Pharmacol Pharmacology Ethnopharmacological relevance: Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities. Aim of the Study: The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of S. glutinosa extracts, as well as the in vivo anti-inflammatory activity. Materials and Methods: Infusions and 70% (v:v) ethanol solution extracts of S. glutinosa stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MS(n) method, and total phenolic and flavonoid content, as well as in vitro antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of in vivo effects of the extracts, using diclofenac as an anti-inflammatory control. Results: The highest inhibitory α-glucosidase activity was determined to be IC(50) = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI(50) = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. In vivo administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production. Conclusion: Our findings indicate that S. glutinosa extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By in vivo administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from S. glutinosa. Frontiers Media S.A. 2022-08-19 /pmc/articles/PMC9437640/ /pubmed/36059937 http://dx.doi.org/10.3389/fphar.2022.975800 Text en Copyright © 2022 Nicolescu, Babotă, Ilea, Dias, Calhelha, Gavrilaș, Rocchetti, Crișan, Mocan, Barros and Pârvu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Nicolescu, Alexandru
Babotă, Mihai
Ilea, Maria
Dias, Maria Inês
Calhelha, Ricardo C.
Gavrilaș, Laura
Rocchetti, Gabriele
Crișan, Gianina
Mocan, Andrei
Barros, Lillian
Pârvu, Alina Elena
Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title_full Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title_fullStr Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title_full_unstemmed Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title_short Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
title_sort potential therapeutic applications of infusions and hydroalcoholic extracts of romanian glutinous sage (salvia glutinosa l.)
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437640/
https://www.ncbi.nlm.nih.gov/pubmed/36059937
http://dx.doi.org/10.3389/fphar.2022.975800
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