Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans

Proximity-dependent protein labeling provides a powerful in vivo strategy to characterize the interactomes of specific proteins. We previously optimized a proximity labeling protocol for Caenorhabditis elegans using the highly active biotin ligase TurboID. A significant constraint on the sensitivity...

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Detalles Bibliográficos
Autores principales: Artan, Murat, Hartl, Markus, Chen, Weiqiang, de Bono, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437848/
https://www.ncbi.nlm.nih.gov/pubmed/35933017
http://dx.doi.org/10.1016/j.jbc.2022.102343

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