Cargando…
Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can b...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438371/ https://www.ncbi.nlm.nih.gov/pubmed/36056449 http://dx.doi.org/10.1186/s13567-022-01084-3 |
_version_ | 1784781813808365568 |
---|---|
author | Te, Nigeer Rodon, Jordi Creve, Rhea Pérez, Mónica Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert |
author_facet | Te, Nigeer Rodon, Jordi Creve, Rhea Pérez, Mónica Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert |
author_sort | Te, Nigeer |
collection | PubMed |
description | Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10(3) TCID(50)/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01084-3. |
format | Online Article Text |
id | pubmed-9438371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94383712022-09-02 Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection Te, Nigeer Rodon, Jordi Creve, Rhea Pérez, Mónica Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert Vet Res Short Report Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10(3) TCID(50)/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01084-3. BioMed Central 2022-09-02 2022 /pmc/articles/PMC9438371/ /pubmed/36056449 http://dx.doi.org/10.1186/s13567-022-01084-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Te, Nigeer Rodon, Jordi Creve, Rhea Pérez, Mónica Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title | Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title_full | Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title_fullStr | Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title_full_unstemmed | Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title_short | Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection |
title_sort | evaluation of alpaca tracheal explants as an ex vivo model for the study of middle east respiratory syndrome coronavirus (mers-cov) infection |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438371/ https://www.ncbi.nlm.nih.gov/pubmed/36056449 http://dx.doi.org/10.1186/s13567-022-01084-3 |
work_keys_str_mv | AT tenigeer evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT rodonjordi evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT creverhea evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT perezmonica evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT segalesjoaquim evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT vergaraalertjulia evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection AT bensaidalbert evaluationofalpacatrachealexplantsasanexvivomodelforthestudyofmiddleeastrespiratorysyndromecoronavirusmerscovinfection |