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Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection

Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can b...

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Autores principales: Te, Nigeer, Rodon, Jordi, Creve, Rhea, Pérez, Mónica, Segalés, Joaquim, Vergara-Alert, Júlia, Bensaid, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438371/
https://www.ncbi.nlm.nih.gov/pubmed/36056449
http://dx.doi.org/10.1186/s13567-022-01084-3
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author Te, Nigeer
Rodon, Jordi
Creve, Rhea
Pérez, Mónica
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
author_facet Te, Nigeer
Rodon, Jordi
Creve, Rhea
Pérez, Mónica
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
author_sort Te, Nigeer
collection PubMed
description Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10(3) TCID(50)/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01084-3.
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spelling pubmed-94383712022-09-02 Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection Te, Nigeer Rodon, Jordi Creve, Rhea Pérez, Mónica Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert Vet Res Short Report Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 10(3) TCID(50)/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-022-01084-3. BioMed Central 2022-09-02 2022 /pmc/articles/PMC9438371/ /pubmed/36056449 http://dx.doi.org/10.1186/s13567-022-01084-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Te, Nigeer
Rodon, Jordi
Creve, Rhea
Pérez, Mónica
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title_full Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title_fullStr Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title_full_unstemmed Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title_short Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
title_sort evaluation of alpaca tracheal explants as an ex vivo model for the study of middle east respiratory syndrome coronavirus (mers-cov) infection
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438371/
https://www.ncbi.nlm.nih.gov/pubmed/36056449
http://dx.doi.org/10.1186/s13567-022-01084-3
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