Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022

The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and v...

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Detalles Bibliográficos
Autores principales: Paran, Nir, Yahalom-Ronen, Yfat, Shifman, Ohad, Lazar, Shirley, Ben-Ami, Ronen, Yakubovsky, Michal, Levy, Itzchak, Wieder-Feinsod, Anat, Amit, Sharon, Katzir, Michal, Carmi-Oren, Noga, Levcovich, Ariela, Hershman-Sarafov, Mirit, Paz, Alona, Thomas, Rebecca, Tamir, Hadas, Cherry-Mimran, Lilach, Erez, Noam, Melamed, Sharon, Barlev-Gross, Moria, Karmi, Shay, Politi, Boaz, Achdout, Hagit, Weiss, Shay, Levy, Haim, Schuster, Ofir, Beth-Din, Adi, Israely, Tomer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438394/
https://www.ncbi.nlm.nih.gov/pubmed/36052723
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.35.2200636
Descripción
Sumario:The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.

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