Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022

The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and v...

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Autores principales: Paran, Nir, Yahalom-Ronen, Yfat, Shifman, Ohad, Lazar, Shirley, Ben-Ami, Ronen, Yakubovsky, Michal, Levy, Itzchak, Wieder-Feinsod, Anat, Amit, Sharon, Katzir, Michal, Carmi-Oren, Noga, Levcovich, Ariela, Hershman-Sarafov, Mirit, Paz, Alona, Thomas, Rebecca, Tamir, Hadas, Cherry-Mimran, Lilach, Erez, Noam, Melamed, Sharon, Barlev-Gross, Moria, Karmi, Shay, Politi, Boaz, Achdout, Hagit, Weiss, Shay, Levy, Haim, Schuster, Ofir, Beth-Din, Adi, Israely, Tomer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438394/
https://www.ncbi.nlm.nih.gov/pubmed/36052723
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.35.2200636
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author Paran, Nir
Yahalom-Ronen, Yfat
Shifman, Ohad
Lazar, Shirley
Ben-Ami, Ronen
Yakubovsky, Michal
Levy, Itzchak
Wieder-Feinsod, Anat
Amit, Sharon
Katzir, Michal
Carmi-Oren, Noga
Levcovich, Ariela
Hershman-Sarafov, Mirit
Paz, Alona
Thomas, Rebecca
Tamir, Hadas
Cherry-Mimran, Lilach
Erez, Noam
Melamed, Sharon
Barlev-Gross, Moria
Karmi, Shay
Politi, Boaz
Achdout, Hagit
Weiss, Shay
Levy, Haim
Schuster, Ofir
Beth-Din, Adi
Israely, Tomer
author_facet Paran, Nir
Yahalom-Ronen, Yfat
Shifman, Ohad
Lazar, Shirley
Ben-Ami, Ronen
Yakubovsky, Michal
Levy, Itzchak
Wieder-Feinsod, Anat
Amit, Sharon
Katzir, Michal
Carmi-Oren, Noga
Levcovich, Ariela
Hershman-Sarafov, Mirit
Paz, Alona
Thomas, Rebecca
Tamir, Hadas
Cherry-Mimran, Lilach
Erez, Noam
Melamed, Sharon
Barlev-Gross, Moria
Karmi, Shay
Politi, Boaz
Achdout, Hagit
Weiss, Shay
Levy, Haim
Schuster, Ofir
Beth-Din, Adi
Israely, Tomer
author_sort Paran, Nir
collection PubMed
description The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.
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spelling pubmed-94383942022-09-19 Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022 Paran, Nir Yahalom-Ronen, Yfat Shifman, Ohad Lazar, Shirley Ben-Ami, Ronen Yakubovsky, Michal Levy, Itzchak Wieder-Feinsod, Anat Amit, Sharon Katzir, Michal Carmi-Oren, Noga Levcovich, Ariela Hershman-Sarafov, Mirit Paz, Alona Thomas, Rebecca Tamir, Hadas Cherry-Mimran, Lilach Erez, Noam Melamed, Sharon Barlev-Gross, Moria Karmi, Shay Politi, Boaz Achdout, Hagit Weiss, Shay Levy, Haim Schuster, Ofir Beth-Din, Adi Israely, Tomer Euro Surveill Rapid Communication The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox. European Centre for Disease Prevention and Control (ECDC) 2022-09-01 /pmc/articles/PMC9438394/ /pubmed/36052723 http://dx.doi.org/10.2807/1560-7917.ES.2022.27.35.2200636 Text en This article is copyright of the authors or their affiliated institutions, 2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Paran, Nir
Yahalom-Ronen, Yfat
Shifman, Ohad
Lazar, Shirley
Ben-Ami, Ronen
Yakubovsky, Michal
Levy, Itzchak
Wieder-Feinsod, Anat
Amit, Sharon
Katzir, Michal
Carmi-Oren, Noga
Levcovich, Ariela
Hershman-Sarafov, Mirit
Paz, Alona
Thomas, Rebecca
Tamir, Hadas
Cherry-Mimran, Lilach
Erez, Noam
Melamed, Sharon
Barlev-Gross, Moria
Karmi, Shay
Politi, Boaz
Achdout, Hagit
Weiss, Shay
Levy, Haim
Schuster, Ofir
Beth-Din, Adi
Israely, Tomer
Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title_full Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title_fullStr Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title_full_unstemmed Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title_short Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
title_sort monkeypox dna levels correlate with virus infectivity in clinical samples, israel, 2022
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438394/
https://www.ncbi.nlm.nih.gov/pubmed/36052723
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.35.2200636
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