Cargando…

Serial small- and wide-angle X-ray scattering with laboratory sources

Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilit...

Descripción completa

Detalles Bibliográficos
Autores principales: Levenstein, Mark A., Robertson, Karen, Turner, Thomas D., Hunter, Liam, O’Brien, Cate, O’Shaughnessy, Cedrick, Kulak, Alexander N., Le Magueres, Pierre, Wojciechowski, Jakub, Mykhaylyk, Oleksandr O., Kapur, Nikil, Meldrum, Fiona C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438489/
https://www.ncbi.nlm.nih.gov/pubmed/36071805
http://dx.doi.org/10.1107/S2052252522007631
Descripción
Sumario:Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.