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Serial small- and wide-angle X-ray scattering with laboratory sources
Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilit...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438489/ https://www.ncbi.nlm.nih.gov/pubmed/36071805 http://dx.doi.org/10.1107/S2052252522007631 |
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author | Levenstein, Mark A. Robertson, Karen Turner, Thomas D. Hunter, Liam O’Brien, Cate O’Shaughnessy, Cedrick Kulak, Alexander N. Le Magueres, Pierre Wojciechowski, Jakub Mykhaylyk, Oleksandr O. Kapur, Nikil Meldrum, Fiona C. |
author_facet | Levenstein, Mark A. Robertson, Karen Turner, Thomas D. Hunter, Liam O’Brien, Cate O’Shaughnessy, Cedrick Kulak, Alexander N. Le Magueres, Pierre Wojciechowski, Jakub Mykhaylyk, Oleksandr O. Kapur, Nikil Meldrum, Fiona C. |
author_sort | Levenstein, Mark A. |
collection | PubMed |
description | Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology. |
format | Online Article Text |
id | pubmed-9438489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-94384892022-09-06 Serial small- and wide-angle X-ray scattering with laboratory sources Levenstein, Mark A. Robertson, Karen Turner, Thomas D. Hunter, Liam O’Brien, Cate O’Shaughnessy, Cedrick Kulak, Alexander N. Le Magueres, Pierre Wojciechowski, Jakub Mykhaylyk, Oleksandr O. Kapur, Nikil Meldrum, Fiona C. IUCrJ Research Letters Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology. International Union of Crystallography 2022-08-13 /pmc/articles/PMC9438489/ /pubmed/36071805 http://dx.doi.org/10.1107/S2052252522007631 Text en © Mark A. Levenstein et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Letters Levenstein, Mark A. Robertson, Karen Turner, Thomas D. Hunter, Liam O’Brien, Cate O’Shaughnessy, Cedrick Kulak, Alexander N. Le Magueres, Pierre Wojciechowski, Jakub Mykhaylyk, Oleksandr O. Kapur, Nikil Meldrum, Fiona C. Serial small- and wide-angle X-ray scattering with laboratory sources |
title | Serial small- and wide-angle X-ray scattering with laboratory sources |
title_full | Serial small- and wide-angle X-ray scattering with laboratory sources |
title_fullStr | Serial small- and wide-angle X-ray scattering with laboratory sources |
title_full_unstemmed | Serial small- and wide-angle X-ray scattering with laboratory sources |
title_short | Serial small- and wide-angle X-ray scattering with laboratory sources |
title_sort | serial small- and wide-angle x-ray scattering with laboratory sources |
topic | Research Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438489/ https://www.ncbi.nlm.nih.gov/pubmed/36071805 http://dx.doi.org/10.1107/S2052252522007631 |
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