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Serial small- and wide-angle X-ray scattering with laboratory sources

Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilit...

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Autores principales: Levenstein, Mark A., Robertson, Karen, Turner, Thomas D., Hunter, Liam, O’Brien, Cate, O’Shaughnessy, Cedrick, Kulak, Alexander N., Le Magueres, Pierre, Wojciechowski, Jakub, Mykhaylyk, Oleksandr O., Kapur, Nikil, Meldrum, Fiona C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438489/
https://www.ncbi.nlm.nih.gov/pubmed/36071805
http://dx.doi.org/10.1107/S2052252522007631
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author Levenstein, Mark A.
Robertson, Karen
Turner, Thomas D.
Hunter, Liam
O’Brien, Cate
O’Shaughnessy, Cedrick
Kulak, Alexander N.
Le Magueres, Pierre
Wojciechowski, Jakub
Mykhaylyk, Oleksandr O.
Kapur, Nikil
Meldrum, Fiona C.
author_facet Levenstein, Mark A.
Robertson, Karen
Turner, Thomas D.
Hunter, Liam
O’Brien, Cate
O’Shaughnessy, Cedrick
Kulak, Alexander N.
Le Magueres, Pierre
Wojciechowski, Jakub
Mykhaylyk, Oleksandr O.
Kapur, Nikil
Meldrum, Fiona C.
author_sort Levenstein, Mark A.
collection PubMed
description Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.
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spelling pubmed-94384892022-09-06 Serial small- and wide-angle X-ray scattering with laboratory sources Levenstein, Mark A. Robertson, Karen Turner, Thomas D. Hunter, Liam O’Brien, Cate O’Shaughnessy, Cedrick Kulak, Alexander N. Le Magueres, Pierre Wojciechowski, Jakub Mykhaylyk, Oleksandr O. Kapur, Nikil Meldrum, Fiona C. IUCrJ Research Letters Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology. International Union of Crystallography 2022-08-13 /pmc/articles/PMC9438489/ /pubmed/36071805 http://dx.doi.org/10.1107/S2052252522007631 Text en © Mark A. Levenstein et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Letters
Levenstein, Mark A.
Robertson, Karen
Turner, Thomas D.
Hunter, Liam
O’Brien, Cate
O’Shaughnessy, Cedrick
Kulak, Alexander N.
Le Magueres, Pierre
Wojciechowski, Jakub
Mykhaylyk, Oleksandr O.
Kapur, Nikil
Meldrum, Fiona C.
Serial small- and wide-angle X-ray scattering with laboratory sources
title Serial small- and wide-angle X-ray scattering with laboratory sources
title_full Serial small- and wide-angle X-ray scattering with laboratory sources
title_fullStr Serial small- and wide-angle X-ray scattering with laboratory sources
title_full_unstemmed Serial small- and wide-angle X-ray scattering with laboratory sources
title_short Serial small- and wide-angle X-ray scattering with laboratory sources
title_sort serial small- and wide-angle x-ray scattering with laboratory sources
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438489/
https://www.ncbi.nlm.nih.gov/pubmed/36071805
http://dx.doi.org/10.1107/S2052252522007631
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