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Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh
Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can potentially repair the spinal cord; however, effects of alginate hydrogels (AHs) on NSPCs rem...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438746/ https://www.ncbi.nlm.nih.gov/pubmed/36072264 http://dx.doi.org/10.1093/rb/rbac057 |
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author | Zhou, Jun Wu, Yaqi Tang, Zhijian Zou, Kaipeng Chen, Juan Lei, Zuowei Wan, Xueyan Liu, Yanchao Zhang, Huaqiu Wang, Yu Blesch, Armin Lei, Ting Liu, Shengwen |
author_facet | Zhou, Jun Wu, Yaqi Tang, Zhijian Zou, Kaipeng Chen, Juan Lei, Zuowei Wan, Xueyan Liu, Yanchao Zhang, Huaqiu Wang, Yu Blesch, Armin Lei, Ting Liu, Shengwen |
author_sort | Zhou, Jun |
collection | PubMed |
description | Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can potentially repair the spinal cord; however, effects of alginate hydrogels (AHs) on NSPCs remain unknown. In this study, we fabricated AHs cross-linked by Ca(2+) and seeded hydrogels with rat embryonic day 14 NSPCs. Immunocytochemistry and electron microscopy show that NSPCs survive, proliferate and differentiate into neurons in vitro within the capillaries. After transplantation into an acute T8 complete spinal cord transection site in adult rats, approximately one-third (38.3%) of grafted cells survive and differentiate into neurons (40.7%), astrocytes (26.6%) and oligodendrocytes (28.4%) at 8 weeks post-grafting. NSPCs promote the growth of host axons within the capillaries in a time-dependent manner. Host axons make synapse-like contacts with NSPC-derived neurons within the hydrogel channels, and graft-derived axons extend into the host white and gray matter making putative synapses. This is paralleled by improved electrophysiological conductivity across the lesion and partial hindlimb locomotor recovery. |
format | Online Article Text |
id | pubmed-9438746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-94387462022-09-06 Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh Zhou, Jun Wu, Yaqi Tang, Zhijian Zou, Kaipeng Chen, Juan Lei, Zuowei Wan, Xueyan Liu, Yanchao Zhang, Huaqiu Wang, Yu Blesch, Armin Lei, Ting Liu, Shengwen Regen Biomater Research Article Alginate capillary hydrogels seeded with differentiated cells can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can potentially repair the spinal cord; however, effects of alginate hydrogels (AHs) on NSPCs remain unknown. In this study, we fabricated AHs cross-linked by Ca(2+) and seeded hydrogels with rat embryonic day 14 NSPCs. Immunocytochemistry and electron microscopy show that NSPCs survive, proliferate and differentiate into neurons in vitro within the capillaries. After transplantation into an acute T8 complete spinal cord transection site in adult rats, approximately one-third (38.3%) of grafted cells survive and differentiate into neurons (40.7%), astrocytes (26.6%) and oligodendrocytes (28.4%) at 8 weeks post-grafting. NSPCs promote the growth of host axons within the capillaries in a time-dependent manner. Host axons make synapse-like contacts with NSPC-derived neurons within the hydrogel channels, and graft-derived axons extend into the host white and gray matter making putative synapses. This is paralleled by improved electrophysiological conductivity across the lesion and partial hindlimb locomotor recovery. Oxford University Press 2022-08-18 /pmc/articles/PMC9438746/ /pubmed/36072264 http://dx.doi.org/10.1093/rb/rbac057 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhou, Jun Wu, Yaqi Tang, Zhijian Zou, Kaipeng Chen, Juan Lei, Zuowei Wan, Xueyan Liu, Yanchao Zhang, Huaqiu Wang, Yu Blesch, Armin Lei, Ting Liu, Shengwen Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title | Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title_full | Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title_fullStr | Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title_full_unstemmed | Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title_short | Alginate hydrogel cross-linked by Ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
title_sort | alginate hydrogel cross-linked by ca(2+) to promote spinal cord neural stem/progenitor cell differentiation and functional recovery after a spinal cord injuryhh |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438746/ https://www.ncbi.nlm.nih.gov/pubmed/36072264 http://dx.doi.org/10.1093/rb/rbac057 |
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