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Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling

Vaping has been reported to cause acute epiglottitis, a life-threatening airway obstruction induced by direct epithelial injury and subsequent inflammatory reaction. Here, we show that we were able to recapitulate this phenomenon in vitro. Exposure of human engineered vocal fold (VF) mucosae to 0.5%...

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Autores principales: Lungova, Vlasta, Wendt, Kristy, Thibeault, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438930/
https://www.ncbi.nlm.nih.gov/pubmed/35770504
http://dx.doi.org/10.1242/dmm.049476
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author Lungova, Vlasta
Wendt, Kristy
Thibeault, Susan L.
author_facet Lungova, Vlasta
Wendt, Kristy
Thibeault, Susan L.
author_sort Lungova, Vlasta
collection PubMed
description Vaping has been reported to cause acute epiglottitis, a life-threatening airway obstruction induced by direct epithelial injury and subsequent inflammatory reaction. Here, we show that we were able to recapitulate this phenomenon in vitro. Exposure of human engineered vocal fold (VF) mucosae to 0.5% and 5% electronic cigarette (e-cigarette) vapor extract (ECVE) for 1 week induced cellular damage of luminal cells, disrupting homeostasis and innate immune responses. Epithelial erosion was likely caused by accumulation of solvents and lipid particles in the cytosol and intercellular spaces, which altered lipid metabolism and plasma membrane properties. Next, we investigated how the mucosal cells responded to the epithelial damage. We withdrew the ECVE from the experimental system and allowed VF mucosae to regenerate for 1, 3 and 7 days, which triggered intense epithelial remodeling. The epithelial changes included expansion of P63 (TP63)-positive basal cells and cytokeratin 14 (KRT14) and laminin subunit α-5 (LAMA5) deposition, which might lead to local basal cell hyperplasia, hyperkeratinization and basement membrane thickening. In summary, vaping presents a threat to VF mucosal health and airway protection, thereby raising further concerns over the safety of e-cigarette use. This article has an associated First Person interview with the first author of the paper.
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spelling pubmed-94389302022-09-06 Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling Lungova, Vlasta Wendt, Kristy Thibeault, Susan L. Dis Model Mech Research Article Vaping has been reported to cause acute epiglottitis, a life-threatening airway obstruction induced by direct epithelial injury and subsequent inflammatory reaction. Here, we show that we were able to recapitulate this phenomenon in vitro. Exposure of human engineered vocal fold (VF) mucosae to 0.5% and 5% electronic cigarette (e-cigarette) vapor extract (ECVE) for 1 week induced cellular damage of luminal cells, disrupting homeostasis and innate immune responses. Epithelial erosion was likely caused by accumulation of solvents and lipid particles in the cytosol and intercellular spaces, which altered lipid metabolism and plasma membrane properties. Next, we investigated how the mucosal cells responded to the epithelial damage. We withdrew the ECVE from the experimental system and allowed VF mucosae to regenerate for 1, 3 and 7 days, which triggered intense epithelial remodeling. The epithelial changes included expansion of P63 (TP63)-positive basal cells and cytokeratin 14 (KRT14) and laminin subunit α-5 (LAMA5) deposition, which might lead to local basal cell hyperplasia, hyperkeratinization and basement membrane thickening. In summary, vaping presents a threat to VF mucosal health and airway protection, thereby raising further concerns over the safety of e-cigarette use. This article has an associated First Person interview with the first author of the paper. The Company of Biologists Ltd 2022-08-23 /pmc/articles/PMC9438930/ /pubmed/35770504 http://dx.doi.org/10.1242/dmm.049476 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Lungova, Vlasta
Wendt, Kristy
Thibeault, Susan L.
Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title_full Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title_fullStr Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title_full_unstemmed Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title_short Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
title_sort exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438930/
https://www.ncbi.nlm.nih.gov/pubmed/35770504
http://dx.doi.org/10.1242/dmm.049476
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