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Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection
The spreading of coronavirus from contacting surfaces and aerosols created a pandemic around the world. To prevent the transmission of SARS-CoV-2 virus and other contagious microbes, disinfection of contacting surfaces is necessary. In this study, a disinfection box equipped with infrared (IR) radia...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9439869/ https://www.ncbi.nlm.nih.gov/pubmed/36063893 http://dx.doi.org/10.1016/j.ijbiomac.2022.08.197 |
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author | Mahanta, Nilkamal Sharma, Swati Sharma, Laipubam Gayatri Pandey, Lalit M. Dixit, Uday Shanker |
author_facet | Mahanta, Nilkamal Sharma, Swati Sharma, Laipubam Gayatri Pandey, Lalit M. Dixit, Uday Shanker |
author_sort | Mahanta, Nilkamal |
collection | PubMed |
description | The spreading of coronavirus from contacting surfaces and aerosols created a pandemic around the world. To prevent the transmission of SARS-CoV-2 virus and other contagious microbes, disinfection of contacting surfaces is necessary. In this study, a disinfection box equipped with infrared (IR) radiation heating and ultraviolet-C (UV-C) radiation is designed and tested for its disinfection ability against pathogenic bacteria and SARS-CoV-2 spike protein. The killing of a Gram-positive, namely, S. aureus and a Gram-negative namely, S. typhi bacteria was studied followed by the inactivation of the spike protein. The experimental parameters were optimized using a statistical tool. For the broad-spectrum antibacterial activity, the optimum condition was holding at 65.61 °C for 13.54 min. The killing of the bacterial pathogen occurred via rupturing the cell walls as depicted by electron microscopy. Further, the unfolding of SARS-CoV-2 spike protein and RNase A was studied under IR and UV-C irradiations at the aforesaid optimized condition. The unfolding of both the proteins was confirmed by changes in the secondary structure, particularly an increase in β-sheets and a decrease in α-helixes. Remarkably, the higher penetration depth of IR waves up to subcutaneous tissue resulted in lower optimum disinfection temperature, <70 °C in vogue. Thus, the combined UV-C and IR radiation is effective in killing the pathogenic bacteria and denaturing the glycoproteins. |
format | Online Article Text |
id | pubmed-9439869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94398692022-09-06 Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection Mahanta, Nilkamal Sharma, Swati Sharma, Laipubam Gayatri Pandey, Lalit M. Dixit, Uday Shanker Int J Biol Macromol Article The spreading of coronavirus from contacting surfaces and aerosols created a pandemic around the world. To prevent the transmission of SARS-CoV-2 virus and other contagious microbes, disinfection of contacting surfaces is necessary. In this study, a disinfection box equipped with infrared (IR) radiation heating and ultraviolet-C (UV-C) radiation is designed and tested for its disinfection ability against pathogenic bacteria and SARS-CoV-2 spike protein. The killing of a Gram-positive, namely, S. aureus and a Gram-negative namely, S. typhi bacteria was studied followed by the inactivation of the spike protein. The experimental parameters were optimized using a statistical tool. For the broad-spectrum antibacterial activity, the optimum condition was holding at 65.61 °C for 13.54 min. The killing of the bacterial pathogen occurred via rupturing the cell walls as depicted by electron microscopy. Further, the unfolding of SARS-CoV-2 spike protein and RNase A was studied under IR and UV-C irradiations at the aforesaid optimized condition. The unfolding of both the proteins was confirmed by changes in the secondary structure, particularly an increase in β-sheets and a decrease in α-helixes. Remarkably, the higher penetration depth of IR waves up to subcutaneous tissue resulted in lower optimum disinfection temperature, <70 °C in vogue. Thus, the combined UV-C and IR radiation is effective in killing the pathogenic bacteria and denaturing the glycoproteins. Elsevier B.V. 2022-11-30 2022-09-03 /pmc/articles/PMC9439869/ /pubmed/36063893 http://dx.doi.org/10.1016/j.ijbiomac.2022.08.197 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mahanta, Nilkamal Sharma, Swati Sharma, Laipubam Gayatri Pandey, Lalit M. Dixit, Uday Shanker Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title | Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title_full | Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title_fullStr | Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title_full_unstemmed | Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title_short | Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection |
title_sort | unfolding of the sars-cov-2 spike protein through infrared and ultraviolet-c radiation based disinfection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9439869/ https://www.ncbi.nlm.nih.gov/pubmed/36063893 http://dx.doi.org/10.1016/j.ijbiomac.2022.08.197 |
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