Cargando…

A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis

In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filamen...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Ting, Xiao, Yanhong, Huang, Bo, Ran, Mao-Jiu, Duan, Xin, Wang, Yu-Feng, Lu, Yuzhen, Yu, Xiao-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440207/
https://www.ncbi.nlm.nih.gov/pubmed/36056003
http://dx.doi.org/10.1038/s41419-022-05195-9
_version_ 1784782286555709440
author Zhao, Ting
Xiao, Yanhong
Huang, Bo
Ran, Mao-Jiu
Duan, Xin
Wang, Yu-Feng
Lu, Yuzhen
Yu, Xiao-Qiang
author_facet Zhao, Ting
Xiao, Yanhong
Huang, Bo
Ran, Mao-Jiu
Duan, Xin
Wang, Yu-Feng
Lu, Yuzhen
Yu, Xiao-Qiang
author_sort Zhao, Ting
collection PubMed
description In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filaments (TFs) are formed, but the underlying mechanism for the development of TFs remains largely unknown. Here we report that transcription factor longitudinals lacking (Lola) is essential for ovary morphogenesis. We showed that Lola protein was expressed abundantly in TFCs and CCs, although also in other cells, and lola was required for the establishment of niche during larval stage. Importantly, we found that knockdown expression of lola induced apoptosis in adult ovary, and that lola affected adult ovary morphogenesis by suppressing expression of Regulator of cullins 1b (Roc1b), an apoptosis-related gene that regulates caspase activation during spermatogenesis. These findings significantly expand our understanding of the mechanisms controlling niche establishment and adult oogenesis in Drosophila.
format Online
Article
Text
id pubmed-9440207
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-94402072022-09-04 A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis Zhao, Ting Xiao, Yanhong Huang, Bo Ran, Mao-Jiu Duan, Xin Wang, Yu-Feng Lu, Yuzhen Yu, Xiao-Qiang Cell Death Dis Article In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filaments (TFs) are formed, but the underlying mechanism for the development of TFs remains largely unknown. Here we report that transcription factor longitudinals lacking (Lola) is essential for ovary morphogenesis. We showed that Lola protein was expressed abundantly in TFCs and CCs, although also in other cells, and lola was required for the establishment of niche during larval stage. Importantly, we found that knockdown expression of lola induced apoptosis in adult ovary, and that lola affected adult ovary morphogenesis by suppressing expression of Regulator of cullins 1b (Roc1b), an apoptosis-related gene that regulates caspase activation during spermatogenesis. These findings significantly expand our understanding of the mechanisms controlling niche establishment and adult oogenesis in Drosophila. Nature Publishing Group UK 2022-09-02 /pmc/articles/PMC9440207/ /pubmed/36056003 http://dx.doi.org/10.1038/s41419-022-05195-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhao, Ting
Xiao, Yanhong
Huang, Bo
Ran, Mao-Jiu
Duan, Xin
Wang, Yu-Feng
Lu, Yuzhen
Yu, Xiao-Qiang
A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title_full A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title_fullStr A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title_full_unstemmed A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title_short A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
title_sort dual role of lola in drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440207/
https://www.ncbi.nlm.nih.gov/pubmed/36056003
http://dx.doi.org/10.1038/s41419-022-05195-9
work_keys_str_mv AT zhaoting adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT xiaoyanhong adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT huangbo adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT ranmaojiu adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT duanxin adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT wangyufeng adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT luyuzhen adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT yuxiaoqiang adualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT zhaoting dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT xiaoyanhong dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT huangbo dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT ranmaojiu dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT duanxin dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT wangyufeng dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT luyuzhen dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis
AT yuxiaoqiang dualroleoflolaindrosophilaovarydevelopmentregulatingstemcellnicheestablishmentandrepressingapoptosis