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No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model

With the rapid development of alternative methods based on the spirit of animal welfare, the publications of animal studies evaluating endpoints such as cancer have been extremely reduced. We performed a 26-week inhalation exposure studies of titanium dioxide nanoparticles (TiO(2) NPs) using CByB6F1...

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Autores principales: Yamano, Shotaro, Takeda, Tomoki, Goto, Yuko, Hirai, Shigeyuki, Furukawa, Yusuke, Kikuchi, Yoshinori, Kasai, Tatsuya, Misumi, Kyohei, Suzuki, Masaaki, Takanobu, Kenji, Senoh, Hideki, Saito, Misae, Kondo, Hitomi, Umeda, Yumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440215/
https://www.ncbi.nlm.nih.gov/pubmed/36056156
http://dx.doi.org/10.1038/s41598-022-19139-y
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author Yamano, Shotaro
Takeda, Tomoki
Goto, Yuko
Hirai, Shigeyuki
Furukawa, Yusuke
Kikuchi, Yoshinori
Kasai, Tatsuya
Misumi, Kyohei
Suzuki, Masaaki
Takanobu, Kenji
Senoh, Hideki
Saito, Misae
Kondo, Hitomi
Umeda, Yumi
author_facet Yamano, Shotaro
Takeda, Tomoki
Goto, Yuko
Hirai, Shigeyuki
Furukawa, Yusuke
Kikuchi, Yoshinori
Kasai, Tatsuya
Misumi, Kyohei
Suzuki, Masaaki
Takanobu, Kenji
Senoh, Hideki
Saito, Misae
Kondo, Hitomi
Umeda, Yumi
author_sort Yamano, Shotaro
collection PubMed
description With the rapid development of alternative methods based on the spirit of animal welfare, the publications of animal studies evaluating endpoints such as cancer have been extremely reduced. We performed a 26-week inhalation exposure studies of titanium dioxide nanoparticles (TiO(2) NPs) using CByB6F1-Tg(HRAS)2Jic (rasH2) mice model for detecting carcinogenicity. Male and female rasH2 mice were exposed to 2, 8 or 32 mg/m(3) of TiO(2) NPs for 6 h/day, 5 days/week for 26 weeks. All tissues and blood were collected and subjected to biological and histopathological analyses. TiO(2) NPs exposure induced deposition of particles in lungs in a dose-dependent manner in each exposure group. Exposure to TiO(2) NPs, as well as other organs, did not increase the incidence of lung tumors in any group, and pulmonary fibrosis and pre-neoplastic lesions were not observed in all groups. Finally, the cell proliferative activity of alveolar epithelial type 2 cells was examined, and it was not increased by exposure to TiO(2) NPs. This is the first report showing the lack of pulmonary fibrogenicity and carcinogenicity (no evidence of carcinogenic activity) of TiO(2) NPs in 26-week inhalation study in rasH2 mice exposed up to 32 mg/m(3), which is considered to be a high concentration.
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spelling pubmed-94402152022-09-04 No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model Yamano, Shotaro Takeda, Tomoki Goto, Yuko Hirai, Shigeyuki Furukawa, Yusuke Kikuchi, Yoshinori Kasai, Tatsuya Misumi, Kyohei Suzuki, Masaaki Takanobu, Kenji Senoh, Hideki Saito, Misae Kondo, Hitomi Umeda, Yumi Sci Rep Article With the rapid development of alternative methods based on the spirit of animal welfare, the publications of animal studies evaluating endpoints such as cancer have been extremely reduced. We performed a 26-week inhalation exposure studies of titanium dioxide nanoparticles (TiO(2) NPs) using CByB6F1-Tg(HRAS)2Jic (rasH2) mice model for detecting carcinogenicity. Male and female rasH2 mice were exposed to 2, 8 or 32 mg/m(3) of TiO(2) NPs for 6 h/day, 5 days/week for 26 weeks. All tissues and blood were collected and subjected to biological and histopathological analyses. TiO(2) NPs exposure induced deposition of particles in lungs in a dose-dependent manner in each exposure group. Exposure to TiO(2) NPs, as well as other organs, did not increase the incidence of lung tumors in any group, and pulmonary fibrosis and pre-neoplastic lesions were not observed in all groups. Finally, the cell proliferative activity of alveolar epithelial type 2 cells was examined, and it was not increased by exposure to TiO(2) NPs. This is the first report showing the lack of pulmonary fibrogenicity and carcinogenicity (no evidence of carcinogenic activity) of TiO(2) NPs in 26-week inhalation study in rasH2 mice exposed up to 32 mg/m(3), which is considered to be a high concentration. Nature Publishing Group UK 2022-09-02 /pmc/articles/PMC9440215/ /pubmed/36056156 http://dx.doi.org/10.1038/s41598-022-19139-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yamano, Shotaro
Takeda, Tomoki
Goto, Yuko
Hirai, Shigeyuki
Furukawa, Yusuke
Kikuchi, Yoshinori
Kasai, Tatsuya
Misumi, Kyohei
Suzuki, Masaaki
Takanobu, Kenji
Senoh, Hideki
Saito, Misae
Kondo, Hitomi
Umeda, Yumi
No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title_full No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title_fullStr No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title_full_unstemmed No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title_short No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model
title_sort no evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rash2 mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440215/
https://www.ncbi.nlm.nih.gov/pubmed/36056156
http://dx.doi.org/10.1038/s41598-022-19139-y
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