Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome

Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8(+) TILs in tumors by single-cell RNA-seq paired to VDJ profil...

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Detalles Bibliográficos
Autores principales: Anadon, Carmen M., Zhang, Chaomei, Wang, Xuefeng, Cen, Ling, Conejo-Garcia, Jose R., Yu, Xiaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440482/
https://www.ncbi.nlm.nih.gov/pubmed/36065294
http://dx.doi.org/10.1016/j.xpro.2022.101649
Descripción
Sumario:Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8(+) TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).

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