Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome

Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8(+) TILs in tumors by single-cell RNA-seq paired to VDJ profil...

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Autores principales: Anadon, Carmen M., Zhang, Chaomei, Wang, Xuefeng, Cen, Ling, Conejo-Garcia, Jose R., Yu, Xiaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440482/
https://www.ncbi.nlm.nih.gov/pubmed/36065294
http://dx.doi.org/10.1016/j.xpro.2022.101649
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author Anadon, Carmen M.
Zhang, Chaomei
Wang, Xuefeng
Cen, Ling
Conejo-Garcia, Jose R.
Yu, Xiaoqing
author_facet Anadon, Carmen M.
Zhang, Chaomei
Wang, Xuefeng
Cen, Ling
Conejo-Garcia, Jose R.
Yu, Xiaoqing
author_sort Anadon, Carmen M.
collection PubMed
description Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8(+) TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).
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spelling pubmed-94404822022-09-04 Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome Anadon, Carmen M. Zhang, Chaomei Wang, Xuefeng Cen, Ling Conejo-Garcia, Jose R. Yu, Xiaoqing STAR Protoc Protocol Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8(+) TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022). Elsevier 2022-08-26 /pmc/articles/PMC9440482/ /pubmed/36065294 http://dx.doi.org/10.1016/j.xpro.2022.101649 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Anadon, Carmen M.
Zhang, Chaomei
Wang, Xuefeng
Cen, Ling
Conejo-Garcia, Jose R.
Yu, Xiaoqing
Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title_full Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title_fullStr Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title_full_unstemmed Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title_short Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
title_sort protocol for the isolation of cd8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440482/
https://www.ncbi.nlm.nih.gov/pubmed/36065294
http://dx.doi.org/10.1016/j.xpro.2022.101649
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