Enhancement of Congo Red-Neomycin Resonance Rayleigh Scattering by Dodecyl Trimethyl Ammonium Bromide and its Application

A simple, rapid, and convenient method for the determination of neomycin based on the ion association method was proposed. In Britton–Robinson buffer solution, neomycin could react with Congo red to form an ionic association, which in turn reacted with dodecyl trimethyl ammonium bromide to form a ternary ionic association. The three were combined in a 1 : 1 : 1 ratio, which significantly enhanced the resonant Rayleigh scattering intensity at 468 nm. The obtained resonant Rayleigh scattering sensor showed a linear relationship with neomycin in the range of 0.07∼1 μg·mL(−1). The limit of detection was 0.02 μg·mL(−1), and the limit of quantification was 0.037 μg·mL(−1). The experimental conditions were optimized. The method was verified based on the ICH rule. The established method could be applied to the analysis of the acceptable recovery rate of neomycin in powdered veterinary drugs.