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Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks

Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of build...

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Autores principales: Ignea, Codruta, Raadam, Morten H., Koutsaviti, Aikaterini, Zhao, Yong, Duan, Yao-Tao, Harizani, Maria, Miettinen, Karel, Georgantea, Panagiota, Rosenfeldt, Mads, Viejo-Ledesma, Sara E., Petersen, Mikael A., Bredie, Wender L. P., Staerk, Dan, Roussis, Vassilios, Ioannou, Efstathia, Kampranis, Sotirios C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440906/
https://www.ncbi.nlm.nih.gov/pubmed/36057727
http://dx.doi.org/10.1038/s41467-022-32921-w
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author Ignea, Codruta
Raadam, Morten H.
Koutsaviti, Aikaterini
Zhao, Yong
Duan, Yao-Tao
Harizani, Maria
Miettinen, Karel
Georgantea, Panagiota
Rosenfeldt, Mads
Viejo-Ledesma, Sara E.
Petersen, Mikael A.
Bredie, Wender L. P.
Staerk, Dan
Roussis, Vassilios
Ioannou, Efstathia
Kampranis, Sotirios C.
author_facet Ignea, Codruta
Raadam, Morten H.
Koutsaviti, Aikaterini
Zhao, Yong
Duan, Yao-Tao
Harizani, Maria
Miettinen, Karel
Georgantea, Panagiota
Rosenfeldt, Mads
Viejo-Ledesma, Sara E.
Petersen, Mikael A.
Bredie, Wender L. P.
Staerk, Dan
Roussis, Vassilios
Ioannou, Efstathia
Kampranis, Sotirios C.
author_sort Ignea, Codruta
collection PubMed
description Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of building blocks. Engineering organisms to synthesize alternative building blocks will bypass this limitation and enable the sustainable production of molecules with non-canonical chemical structures, expanding the possible applications. Herein, we focus on isoprenoids and combine synthetic biology with protein engineering to construct yeast cells that synthesize 10 non-canonical isoprenoid building blocks with 16 carbon atoms. We identify suitable terpene synthases to convert these building blocks into C(16) scaffolds and a cytochrome P450 to decorate the terpene scaffolds and produce different oxygenated compounds. Thus, we reconstruct the modular structure of terpene biosynthesis on 16-carbon backbones, synthesizing 28 different non-canonical terpenes, some of which have interesting odorant properties.
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spelling pubmed-94409062022-09-05 Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks Ignea, Codruta Raadam, Morten H. Koutsaviti, Aikaterini Zhao, Yong Duan, Yao-Tao Harizani, Maria Miettinen, Karel Georgantea, Panagiota Rosenfeldt, Mads Viejo-Ledesma, Sara E. Petersen, Mikael A. Bredie, Wender L. P. Staerk, Dan Roussis, Vassilios Ioannou, Efstathia Kampranis, Sotirios C. Nat Commun Article Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of building blocks. Engineering organisms to synthesize alternative building blocks will bypass this limitation and enable the sustainable production of molecules with non-canonical chemical structures, expanding the possible applications. Herein, we focus on isoprenoids and combine synthetic biology with protein engineering to construct yeast cells that synthesize 10 non-canonical isoprenoid building blocks with 16 carbon atoms. We identify suitable terpene synthases to convert these building blocks into C(16) scaffolds and a cytochrome P450 to decorate the terpene scaffolds and produce different oxygenated compounds. Thus, we reconstruct the modular structure of terpene biosynthesis on 16-carbon backbones, synthesizing 28 different non-canonical terpenes, some of which have interesting odorant properties. Nature Publishing Group UK 2022-09-03 /pmc/articles/PMC9440906/ /pubmed/36057727 http://dx.doi.org/10.1038/s41467-022-32921-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ignea, Codruta
Raadam, Morten H.
Koutsaviti, Aikaterini
Zhao, Yong
Duan, Yao-Tao
Harizani, Maria
Miettinen, Karel
Georgantea, Panagiota
Rosenfeldt, Mads
Viejo-Ledesma, Sara E.
Petersen, Mikael A.
Bredie, Wender L. P.
Staerk, Dan
Roussis, Vassilios
Ioannou, Efstathia
Kampranis, Sotirios C.
Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title_full Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title_fullStr Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title_full_unstemmed Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title_short Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
title_sort expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440906/
https://www.ncbi.nlm.nih.gov/pubmed/36057727
http://dx.doi.org/10.1038/s41467-022-32921-w
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