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Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study
BACKGROUND: As patient exposure to ionizing radiation raises concern about malignancy risks, this study evaluated the effect of ionizing radiation on patients undergoing myocardial perfusion imaging (MPI) using the comet assay, a method for detection of DNA damage. METHODS: Patients without cancer,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441099/ https://www.ncbi.nlm.nih.gov/pubmed/36057570 http://dx.doi.org/10.1186/s12872-022-02839-8 |
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author | Arpini, Anna Paula De Lorenzo, Andrea Moritz, Aniele Pereira, Julia Passarelli Dias, Glauber Monteiro |
author_facet | Arpini, Anna Paula De Lorenzo, Andrea Moritz, Aniele Pereira, Julia Passarelli Dias, Glauber Monteiro |
author_sort | Arpini, Anna Paula |
collection | PubMed |
description | BACKGROUND: As patient exposure to ionizing radiation raises concern about malignancy risks, this study evaluated the effect of ionizing radiation on patients undergoing myocardial perfusion imaging (MPI) using the comet assay, a method for detection of DNA damage. METHODS: Patients without cancer, acute or autoimmune diseases, recent surgery or trauma, were studied. Gated single-photon myocardial perfusion imaging was performed with Tc-99m sestamibi. Peripheral blood was collected before radiotracer injection at rest and 60–90 min after injection. Single-cell gel electrophoresis (comet assay) was performed with blood lymphocytes to detect strand breaks, which determine a “comet tail” of variable size, visually scored by 3 observers in a fluorescence microscope after staining (0: no damage, no tail; 1: small damage; 2: large damage; 3: full damage). A damage index was calculated as a weighted average of the cell scores. RESULTS: Among the 29 individuals included in the analysis, age was 65.3 ± 9.9 years and 18 (62.1%) were male. The injected radiotracer dose was 880.6 ± 229.4 MBq. Most cells (approximately 70%) remained without DNA fragmentation (class 0) after tracer injection. There were nonsignificant increases of classes 1 and 2 of damage. Class 3 was the least frequent both before and after radiotracer injection, but displayed a significant, 44% increase after injection. CONCLUSION: While lymphocytes mostly remained in class 0, an increase in class 3 DNA damage was detected. This may suggest that, despite a probable lack of biologically relevant DNA damage, there is still a need for tracer dose reductions in MPI. |
format | Online Article Text |
id | pubmed-9441099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94410992022-09-05 Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study Arpini, Anna Paula De Lorenzo, Andrea Moritz, Aniele Pereira, Julia Passarelli Dias, Glauber Monteiro BMC Cardiovasc Disord Research BACKGROUND: As patient exposure to ionizing radiation raises concern about malignancy risks, this study evaluated the effect of ionizing radiation on patients undergoing myocardial perfusion imaging (MPI) using the comet assay, a method for detection of DNA damage. METHODS: Patients without cancer, acute or autoimmune diseases, recent surgery or trauma, were studied. Gated single-photon myocardial perfusion imaging was performed with Tc-99m sestamibi. Peripheral blood was collected before radiotracer injection at rest and 60–90 min after injection. Single-cell gel electrophoresis (comet assay) was performed with blood lymphocytes to detect strand breaks, which determine a “comet tail” of variable size, visually scored by 3 observers in a fluorescence microscope after staining (0: no damage, no tail; 1: small damage; 2: large damage; 3: full damage). A damage index was calculated as a weighted average of the cell scores. RESULTS: Among the 29 individuals included in the analysis, age was 65.3 ± 9.9 years and 18 (62.1%) were male. The injected radiotracer dose was 880.6 ± 229.4 MBq. Most cells (approximately 70%) remained without DNA fragmentation (class 0) after tracer injection. There were nonsignificant increases of classes 1 and 2 of damage. Class 3 was the least frequent both before and after radiotracer injection, but displayed a significant, 44% increase after injection. CONCLUSION: While lymphocytes mostly remained in class 0, an increase in class 3 DNA damage was detected. This may suggest that, despite a probable lack of biologically relevant DNA damage, there is still a need for tracer dose reductions in MPI. BioMed Central 2022-09-03 /pmc/articles/PMC9441099/ /pubmed/36057570 http://dx.doi.org/10.1186/s12872-022-02839-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Arpini, Anna Paula De Lorenzo, Andrea Moritz, Aniele Pereira, Julia Passarelli Dias, Glauber Monteiro Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title | Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title_full | Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title_fullStr | Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title_full_unstemmed | Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title_short | Evaluation of DNA damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
title_sort | evaluation of dna damage induced by ionizing radiation from myocardial perfusion imaging: a pilot study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441099/ https://www.ncbi.nlm.nih.gov/pubmed/36057570 http://dx.doi.org/10.1186/s12872-022-02839-8 |
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