Cargando…

Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance

OBJECTIVE: This study aims to explore the serum levels of neuregulin 4 (NRG4), afamin (AFM), and serpin family B member 1 (SERPINB1) in gestational diabetes mellitus (GDM) patients and their relationship with insulin resistance. METHOD: Serum levels of AFM, SERPINB1, and NRG4 were measured in GDM (n...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qian, Li, Chunmei, Jin, Jing, Shen, Yang, Wang, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441345/
https://www.ncbi.nlm.nih.gov/pubmed/36072413
http://dx.doi.org/10.1155/2022/2829662
_version_ 1784782550874456064
author Li, Qian
Li, Chunmei
Jin, Jing
Shen, Yang
Wang, Mei
author_facet Li, Qian
Li, Chunmei
Jin, Jing
Shen, Yang
Wang, Mei
author_sort Li, Qian
collection PubMed
description OBJECTIVE: This study aims to explore the serum levels of neuregulin 4 (NRG4), afamin (AFM), and serpin family B member 1 (SERPINB1) in gestational diabetes mellitus (GDM) patients and their relationship with insulin resistance. METHOD: Serum levels of AFM, SERPINB1, and NRG4 were measured in GDM (n = 58), and non-GDM women (n = 60) using enzyme-linked immunosorbent assay (ELISA) kits. Besides, the serum insulin and glucose levels were also measured followed by calculating the homeostatic model assessment of insulin resistance (HOMA-IR). The correlation was performed using the Pearson analysis. RESULTS: The increased serum levels of AFM and SERPINB1 were revealed in GDM patients as compared with non-GDM women, accompanied by the lower NRG4 serum level. ROCs for AFM concentrations showed an AUC of 0.629 (95% CI: 0.527∼0.731), 0.832 (95% CI: 0.754∼0.909) for the SERPINB1 serum level, and 0.626 (95% CI: 0.524∼0.728) for the NRG4 serum level. The threshold was 108.05 mg/L, 8.75 ng/mL, and 96.25 ng/mL of AFM, SERPINB1, and NRG4. Moreover, the combined ROC of AFM, SERPINB1, and NRG4 serum levels showed higher sensitivity (72.41%) and specificity (85.00%) for the diagnosis of GDM (AUC = 0.839; 95% CI: 0.764∼0.913). In GDM patients, the Pearson analysis revealed a significant correlation between AFM and SERPINB1 (r = 0.776), AFM and NRG4 (r = −0.799), as well as SERPINB1 and NRG4 (r = −0.783). Moreover, AFM and SERPINB1 serum concentrations in GDM patients were positively related to insulin levels, fasting glucose levels, and HOMA-IR values. However, the SERPINB1 serum level was negatively correlated with serum insulin and glucose levels and HOMA-IR. CONCLUSION: Abnormal serum levels of NRG4, AFM, and SERPINB1, as highly sensitive diagnostic tools, are closely related to insulin resistance in GDM patients.
format Online
Article
Text
id pubmed-9441345
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-94413452022-09-06 Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance Li, Qian Li, Chunmei Jin, Jing Shen, Yang Wang, Mei Evid Based Complement Alternat Med Research Article OBJECTIVE: This study aims to explore the serum levels of neuregulin 4 (NRG4), afamin (AFM), and serpin family B member 1 (SERPINB1) in gestational diabetes mellitus (GDM) patients and their relationship with insulin resistance. METHOD: Serum levels of AFM, SERPINB1, and NRG4 were measured in GDM (n = 58), and non-GDM women (n = 60) using enzyme-linked immunosorbent assay (ELISA) kits. Besides, the serum insulin and glucose levels were also measured followed by calculating the homeostatic model assessment of insulin resistance (HOMA-IR). The correlation was performed using the Pearson analysis. RESULTS: The increased serum levels of AFM and SERPINB1 were revealed in GDM patients as compared with non-GDM women, accompanied by the lower NRG4 serum level. ROCs for AFM concentrations showed an AUC of 0.629 (95% CI: 0.527∼0.731), 0.832 (95% CI: 0.754∼0.909) for the SERPINB1 serum level, and 0.626 (95% CI: 0.524∼0.728) for the NRG4 serum level. The threshold was 108.05 mg/L, 8.75 ng/mL, and 96.25 ng/mL of AFM, SERPINB1, and NRG4. Moreover, the combined ROC of AFM, SERPINB1, and NRG4 serum levels showed higher sensitivity (72.41%) and specificity (85.00%) for the diagnosis of GDM (AUC = 0.839; 95% CI: 0.764∼0.913). In GDM patients, the Pearson analysis revealed a significant correlation between AFM and SERPINB1 (r = 0.776), AFM and NRG4 (r = −0.799), as well as SERPINB1 and NRG4 (r = −0.783). Moreover, AFM and SERPINB1 serum concentrations in GDM patients were positively related to insulin levels, fasting glucose levels, and HOMA-IR values. However, the SERPINB1 serum level was negatively correlated with serum insulin and glucose levels and HOMA-IR. CONCLUSION: Abnormal serum levels of NRG4, AFM, and SERPINB1, as highly sensitive diagnostic tools, are closely related to insulin resistance in GDM patients. Hindawi 2022-08-28 /pmc/articles/PMC9441345/ /pubmed/36072413 http://dx.doi.org/10.1155/2022/2829662 Text en Copyright © 2022 Qian Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Qian
Li, Chunmei
Jin, Jing
Shen, Yang
Wang, Mei
Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title_full Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title_fullStr Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title_full_unstemmed Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title_short Clinical Significance of Neuregulin 4, Afamin, and SERPINB1 in Gestational Diabetes Mellitus and Their Relationship with Insulin Resistance
title_sort clinical significance of neuregulin 4, afamin, and serpinb1 in gestational diabetes mellitus and their relationship with insulin resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441345/
https://www.ncbi.nlm.nih.gov/pubmed/36072413
http://dx.doi.org/10.1155/2022/2829662
work_keys_str_mv AT liqian clinicalsignificanceofneuregulin4afaminandserpinb1ingestationaldiabetesmellitusandtheirrelationshipwithinsulinresistance
AT lichunmei clinicalsignificanceofneuregulin4afaminandserpinb1ingestationaldiabetesmellitusandtheirrelationshipwithinsulinresistance
AT jinjing clinicalsignificanceofneuregulin4afaminandserpinb1ingestationaldiabetesmellitusandtheirrelationshipwithinsulinresistance
AT shenyang clinicalsignificanceofneuregulin4afaminandserpinb1ingestationaldiabetesmellitusandtheirrelationshipwithinsulinresistance
AT wangmei clinicalsignificanceofneuregulin4afaminandserpinb1ingestationaldiabetesmellitusandtheirrelationshipwithinsulinresistance