EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway

The aim of this study is to elucidate molecular mechanism by which E1A-like inhibitor of differentiation 3 (EID3) promotes cancer stem cell-like phenotypes in osteosarcoma. Overexpression of EID3 in osteosarcoma cells generated more spherical clones, enhanced the expression of stemness-associated ge...

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Autores principales: Wang, Yan, Luo, Shiyong, Wang, Yuxuan, Yang, Shengbang, Huang, Zhitong, Zhu, Xuejin, Cai, Shanghua, Guo, Qifeng, Zhong, Weide, Liu, Sihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441394/
https://www.ncbi.nlm.nih.gov/pubmed/36071872
http://dx.doi.org/10.1155/2022/5941562
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author Wang, Yan
Luo, Shiyong
Wang, Yuxuan
Yang, Shengbang
Huang, Zhitong
Zhu, Xuejin
Cai, Shanghua
Guo, Qifeng
Zhong, Weide
Liu, Sihong
author_facet Wang, Yan
Luo, Shiyong
Wang, Yuxuan
Yang, Shengbang
Huang, Zhitong
Zhu, Xuejin
Cai, Shanghua
Guo, Qifeng
Zhong, Weide
Liu, Sihong
author_sort Wang, Yan
collection PubMed
description The aim of this study is to elucidate molecular mechanism by which E1A-like inhibitor of differentiation 3 (EID3) promotes cancer stem cell-like phenotypes in osteosarcoma. Overexpression of EID3 in osteosarcoma cells generated more spherical clones, enhanced the expression of stemness-associated genes, and promoted chemoresistance, invasion, and metastasis. Furthermore, osteosarcoma cells overexpressing EID3 had increased ability to grow in suspension as osteospheres with high expression of Sox2 and stem cell marker CD133. In addition, knockdown of EID3 reduced sphere formation and inhibited osteosarcoma cell migration and invasion. RNA sequencing and bioinformatics analysis revealed that PI3K-Akt signaling pathway and MAPK pathway­related genes were enriched in osteosarcoma cells with high expression of EID3. Taken together, EID3 promotes osteosarcoma, and EID3–PI3K-Akt axis is a potential therapeutic target for osteosarcoma treatment.
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spelling pubmed-94413942022-09-06 EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway Wang, Yan Luo, Shiyong Wang, Yuxuan Yang, Shengbang Huang, Zhitong Zhu, Xuejin Cai, Shanghua Guo, Qifeng Zhong, Weide Liu, Sihong Oxid Med Cell Longev Research Article The aim of this study is to elucidate molecular mechanism by which E1A-like inhibitor of differentiation 3 (EID3) promotes cancer stem cell-like phenotypes in osteosarcoma. Overexpression of EID3 in osteosarcoma cells generated more spherical clones, enhanced the expression of stemness-associated genes, and promoted chemoresistance, invasion, and metastasis. Furthermore, osteosarcoma cells overexpressing EID3 had increased ability to grow in suspension as osteospheres with high expression of Sox2 and stem cell marker CD133. In addition, knockdown of EID3 reduced sphere formation and inhibited osteosarcoma cell migration and invasion. RNA sequencing and bioinformatics analysis revealed that PI3K-Akt signaling pathway and MAPK pathway­related genes were enriched in osteosarcoma cells with high expression of EID3. Taken together, EID3 promotes osteosarcoma, and EID3–PI3K-Akt axis is a potential therapeutic target for osteosarcoma treatment. Hindawi 2022-08-28 /pmc/articles/PMC9441394/ /pubmed/36071872 http://dx.doi.org/10.1155/2022/5941562 Text en Copyright © 2022 Yan Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Yan
Luo, Shiyong
Wang, Yuxuan
Yang, Shengbang
Huang, Zhitong
Zhu, Xuejin
Cai, Shanghua
Guo, Qifeng
Zhong, Weide
Liu, Sihong
EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title_full EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title_fullStr EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title_full_unstemmed EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title_short EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
title_sort eid3 promotes cancer stem cell-like phenotypes in osteosarcoma through the activation of pi3k-akt signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441394/
https://www.ncbi.nlm.nih.gov/pubmed/36071872
http://dx.doi.org/10.1155/2022/5941562
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