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Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture

The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been success...

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Autores principales: Crosara, Paulo Fernando Tormin Borges, Vasconcelos, Anilton César, Guimarães, Roberto Eustáquio Santos, Becker, Helena Maria Gonçalves, Becker, Celso Gonçalves, Crosara, Sandra Letícia Reis, Nascimento, Evaldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441983/
https://www.ncbi.nlm.nih.gov/pubmed/16446960
http://dx.doi.org/10.1016/S1808-8694(15)31199-X
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author Crosara, Paulo Fernando Tormin Borges
Vasconcelos, Anilton César
Guimarães, Roberto Eustáquio Santos
Becker, Helena Maria Gonçalves
Becker, Celso Gonçalves
Crosara, Sandra Letícia Reis
Nascimento, Evaldo
author_facet Crosara, Paulo Fernando Tormin Borges
Vasconcelos, Anilton César
Guimarães, Roberto Eustáquio Santos
Becker, Helena Maria Gonçalves
Becker, Celso Gonçalves
Crosara, Sandra Letícia Reis
Nascimento, Evaldo
author_sort Crosara, Paulo Fernando Tormin Borges
collection PubMed
description The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology. Aim: The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps. Study design: case-control. Material and Method: This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method. Results: Reduction in GM-CSF expression on the experimental group at time 24 h (p<= 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p=0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours. Conclusion: This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.
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spelling pubmed-94419832022-09-09 Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture Crosara, Paulo Fernando Tormin Borges Vasconcelos, Anilton César Guimarães, Roberto Eustáquio Santos Becker, Helena Maria Gonçalves Becker, Celso Gonçalves Crosara, Sandra Letícia Reis Nascimento, Evaldo Braz J Otorhinolaryngol Original Article The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology. Aim: The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps. Study design: case-control. Material and Method: This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method. Results: Reduction in GM-CSF expression on the experimental group at time 24 h (p<= 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p=0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours. Conclusion: This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies. Elsevier 2015-10-20 /pmc/articles/PMC9441983/ /pubmed/16446960 http://dx.doi.org/10.1016/S1808-8694(15)31199-X Text en . https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Crosara, Paulo Fernando Tormin Borges
Vasconcelos, Anilton César
Guimarães, Roberto Eustáquio Santos
Becker, Helena Maria Gonçalves
Becker, Celso Gonçalves
Crosara, Sandra Letícia Reis
Nascimento, Evaldo
Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title_full Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title_fullStr Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title_full_unstemmed Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title_short Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
title_sort effect of mitomycin c on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441983/
https://www.ncbi.nlm.nih.gov/pubmed/16446960
http://dx.doi.org/10.1016/S1808-8694(15)31199-X
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