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Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies

Testicular function and structure harmed by ageing. Goal of this research was to assess preventive actions of soy isoflavone oral administration for 8 weeks on testes of old male albino rats, and potential mechanisms of action. Adult control (N = 10) and elderly control (N = 10) rats were fed usual...

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Autor principal: Al-Shaikh, Turki M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442360/
https://www.ncbi.nlm.nih.gov/pubmed/36072015
http://dx.doi.org/10.1016/j.sjbs.2022.103423
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author Al-Shaikh, Turki M.
author_facet Al-Shaikh, Turki M.
author_sort Al-Shaikh, Turki M.
collection PubMed
description Testicular function and structure harmed by ageing. Goal of this research was to assess preventive actions of soy isoflavone oral administration for 8 weeks on testes of old male albino rats, and potential mechanisms of action. Adult control (N = 10) and elderly control (N = 10) rats were fed usual diet, while aged treatment group (N = 10) gave oral 100 mg/kg soy isoflavone daily for 8 weeks. ELISA kits were used to measure testosterone levels and oxidative stress indicators [malonaldehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD)] in serum. Aging produced functional and structural testicular changes and decreased ki67 proliferative marker immunoexpression versus adult control rats due to enhancement of oxidative stress. Soy isoflavone exerted protective effect on testicular function and structure as assessed by increase serum levels of testosterone and preserved histological structure and immune-expression features. These protected effects due to isoflavone antioxidant properties proved by decrease in serum values of MDA, while GSH and SOD were elevated after treatment. These data demonstrated protective effects of isoflavone against age changes in rat testes, by reducing oxidative stress and increasing antioxidants and testicular ki67 proliferative marker immunoexpression.
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spelling pubmed-94423602022-09-06 Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies Al-Shaikh, Turki M. Saudi J Biol Sci Original Article Testicular function and structure harmed by ageing. Goal of this research was to assess preventive actions of soy isoflavone oral administration for 8 weeks on testes of old male albino rats, and potential mechanisms of action. Adult control (N = 10) and elderly control (N = 10) rats were fed usual diet, while aged treatment group (N = 10) gave oral 100 mg/kg soy isoflavone daily for 8 weeks. ELISA kits were used to measure testosterone levels and oxidative stress indicators [malonaldehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD)] in serum. Aging produced functional and structural testicular changes and decreased ki67 proliferative marker immunoexpression versus adult control rats due to enhancement of oxidative stress. Soy isoflavone exerted protective effect on testicular function and structure as assessed by increase serum levels of testosterone and preserved histological structure and immune-expression features. These protected effects due to isoflavone antioxidant properties proved by decrease in serum values of MDA, while GSH and SOD were elevated after treatment. These data demonstrated protective effects of isoflavone against age changes in rat testes, by reducing oxidative stress and increasing antioxidants and testicular ki67 proliferative marker immunoexpression. Elsevier 2022-10 2022-08-18 /pmc/articles/PMC9442360/ /pubmed/36072015 http://dx.doi.org/10.1016/j.sjbs.2022.103423 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Al-Shaikh, Turki M.
Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title_full Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title_fullStr Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title_full_unstemmed Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title_short Role of soy isoflavone in preventing aging changes in rat testis: Biochemical and histological studies
title_sort role of soy isoflavone in preventing aging changes in rat testis: biochemical and histological studies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442360/
https://www.ncbi.nlm.nih.gov/pubmed/36072015
http://dx.doi.org/10.1016/j.sjbs.2022.103423
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