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Occult HCV infection in liver transplanted patients: frequency and consequences

AIM OF THE STUDY: Occult hepatitis C virus (HCV) infection (OCI) is a potential source of relapse after liver transplantation with subsequent graft damage. The aim of the study was to detect OCI in patients with living donor liver transplantation (LDLT) who achieved sustained virological response (S...

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Autores principales: Saad, Zeinab M, Ghany, Wael Abd El, Khalifa, Rofida, Higazi, Aliaa, Al-Shazly, Mostafa, Said, Mohamed, Keryakos, Hesham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442662/
https://www.ncbi.nlm.nih.gov/pubmed/36092754
http://dx.doi.org/10.5114/ceh.2022.115116
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author Saad, Zeinab M
Ghany, Wael Abd El
Khalifa, Rofida
Higazi, Aliaa
Al-Shazly, Mostafa
Said, Mohamed
Keryakos, Hesham
author_facet Saad, Zeinab M
Ghany, Wael Abd El
Khalifa, Rofida
Higazi, Aliaa
Al-Shazly, Mostafa
Said, Mohamed
Keryakos, Hesham
author_sort Saad, Zeinab M
collection PubMed
description AIM OF THE STUDY: Occult hepatitis C virus (HCV) infection (OCI) is a potential source of relapse after liver transplantation with subsequent graft damage. The aim of the study was to detect OCI in patients with living donor liver transplantation (LDLT) who achieved sustained virological response (SVR) after sofosbuvir-based antiviral treatment, and to detect risk factors associated with the development of OCI as well as to determine the effect of direct acting antiviral (DAA) therapy after liver transplantation. MATERIAL AND METHODS: 41 patients with living donor liver transplantation who did not receive DAAs before with recurrent HCV infection who achieved a SVR with sofosbuvir-based therapy for 12-24 weeks were recruited. These patients were tested for OCI by HCV-RNA in peripheral blood mononuclear cells (PBMNCs). Those patients with OCI were followed up every 6 months with alanine aminotransferase (ALT), aspartate aminotransferase (AST), and serum HCV-RNA by PCR for 2 years. RESULTS: 92.7% of treated patients achieved HCV SVR 12 weeks. OCI was detected in 4 patients. After follow-up for 18 months, 3 patients continued to have OCI, but one patient presented with progressive elevation of liver enzymes and developed overt HCV infection with positive HCV-RNA PCR in the serum. This patient was retreated with sofosbuvir 400 mg + ledipasvir 90 mg for 12 weeks with resultant negative HCV-RNA PCR in both serum and PBMNCs in addition to normalization of liver enzymes. CONCLUSIONS: Occult HCV infection is a potential source of HCV relapse after liver transplantation which should be investigated for in PBMNCs or liver biopsy.
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spelling pubmed-94426622022-09-09 Occult HCV infection in liver transplanted patients: frequency and consequences Saad, Zeinab M Ghany, Wael Abd El Khalifa, Rofida Higazi, Aliaa Al-Shazly, Mostafa Said, Mohamed Keryakos, Hesham Clin Exp Hepatol Original Paper AIM OF THE STUDY: Occult hepatitis C virus (HCV) infection (OCI) is a potential source of relapse after liver transplantation with subsequent graft damage. The aim of the study was to detect OCI in patients with living donor liver transplantation (LDLT) who achieved sustained virological response (SVR) after sofosbuvir-based antiviral treatment, and to detect risk factors associated with the development of OCI as well as to determine the effect of direct acting antiviral (DAA) therapy after liver transplantation. MATERIAL AND METHODS: 41 patients with living donor liver transplantation who did not receive DAAs before with recurrent HCV infection who achieved a SVR with sofosbuvir-based therapy for 12-24 weeks were recruited. These patients were tested for OCI by HCV-RNA in peripheral blood mononuclear cells (PBMNCs). Those patients with OCI were followed up every 6 months with alanine aminotransferase (ALT), aspartate aminotransferase (AST), and serum HCV-RNA by PCR for 2 years. RESULTS: 92.7% of treated patients achieved HCV SVR 12 weeks. OCI was detected in 4 patients. After follow-up for 18 months, 3 patients continued to have OCI, but one patient presented with progressive elevation of liver enzymes and developed overt HCV infection with positive HCV-RNA PCR in the serum. This patient was retreated with sofosbuvir 400 mg + ledipasvir 90 mg for 12 weeks with resultant negative HCV-RNA PCR in both serum and PBMNCs in addition to normalization of liver enzymes. CONCLUSIONS: Occult HCV infection is a potential source of HCV relapse after liver transplantation which should be investigated for in PBMNCs or liver biopsy. Termedia Publishing House 2022-04-05 2022-06 /pmc/articles/PMC9442662/ /pubmed/36092754 http://dx.doi.org/10.5114/ceh.2022.115116 Text en Copyright © 2022 Clinical and Experimental Hepatology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) )
spellingShingle Original Paper
Saad, Zeinab M
Ghany, Wael Abd El
Khalifa, Rofida
Higazi, Aliaa
Al-Shazly, Mostafa
Said, Mohamed
Keryakos, Hesham
Occult HCV infection in liver transplanted patients: frequency and consequences
title Occult HCV infection in liver transplanted patients: frequency and consequences
title_full Occult HCV infection in liver transplanted patients: frequency and consequences
title_fullStr Occult HCV infection in liver transplanted patients: frequency and consequences
title_full_unstemmed Occult HCV infection in liver transplanted patients: frequency and consequences
title_short Occult HCV infection in liver transplanted patients: frequency and consequences
title_sort occult hcv infection in liver transplanted patients: frequency and consequences
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442662/
https://www.ncbi.nlm.nih.gov/pubmed/36092754
http://dx.doi.org/10.5114/ceh.2022.115116
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