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Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State
[Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with c...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ https://www.ncbi.nlm.nih.gov/pubmed/36000820 http://dx.doi.org/10.1021/acs.jpclett.2c01974 |
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author | Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford |
author_facet | Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford |
author_sort | Malakar, Partha |
collection | PubMed |
description | [Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with complex curve-crossing kinetics. Herein, we report the emergence of such an indicator. Using pH control over retinal isomer ratios, photoinduced transient absorption is recorded in an inward proton pumping Antarctic microbial rhodopsin (AntR) for 13-cis and all-trans retinal resting states. The all-trans fluorescent state decays with 1 ps exponential kinetics. In contrast, in 13-cis it decays within ∼300 fs accompanied by continuous spectral evolution, indicating ballistic internal conversion. The coherent wave packet nature of 13-cis isomerization in AntR matches published results for bacteriorhodopsin (BR) and Anabaena sensory rhodopsin (ASR), which also accommodate both all-trans and 13-cis retinal resting states, marking the emergence of a first structure–photodynamics indicator which holds for all three tested pigments. |
format | Online Article Text |
id | pubmed-9442786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-94427862022-09-06 Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford J Phys Chem Lett [Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with complex curve-crossing kinetics. Herein, we report the emergence of such an indicator. Using pH control over retinal isomer ratios, photoinduced transient absorption is recorded in an inward proton pumping Antarctic microbial rhodopsin (AntR) for 13-cis and all-trans retinal resting states. The all-trans fluorescent state decays with 1 ps exponential kinetics. In contrast, in 13-cis it decays within ∼300 fs accompanied by continuous spectral evolution, indicating ballistic internal conversion. The coherent wave packet nature of 13-cis isomerization in AntR matches published results for bacteriorhodopsin (BR) and Anabaena sensory rhodopsin (ASR), which also accommodate both all-trans and 13-cis retinal resting states, marking the emergence of a first structure–photodynamics indicator which holds for all three tested pigments. American Chemical Society 2022-08-24 2022-09-01 /pmc/articles/PMC9442786/ /pubmed/36000820 http://dx.doi.org/10.1021/acs.jpclett.2c01974 Text en © 2022 American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title | Bidirectional
Photochemistry of Antarctic Microbial
Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the
13-cis Resting State |
title_full | Bidirectional
Photochemistry of Antarctic Microbial
Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the
13-cis Resting State |
title_fullStr | Bidirectional
Photochemistry of Antarctic Microbial
Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the
13-cis Resting State |
title_full_unstemmed | Bidirectional
Photochemistry of Antarctic Microbial
Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the
13-cis Resting State |
title_short | Bidirectional
Photochemistry of Antarctic Microbial
Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the
13-cis Resting State |
title_sort | bidirectional
photochemistry of antarctic microbial
rhodopsin: emerging trend of ballistic photoisomerization from the
13-cis resting state |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ https://www.ncbi.nlm.nih.gov/pubmed/36000820 http://dx.doi.org/10.1021/acs.jpclett.2c01974 |
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