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A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein–Protein Interactions

[Image: see text] The yeast two-hybrid (Y2H) assay is widely used for protein–protein interaction characterization due to its simplicity and accessibility. However, it may mask changes in affinity caused by mutations or ligand activation due to signal saturation. To overcome this drawback, we modifi...

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Detalles Bibliográficos
Autores principales:	Feuer, Erez, Zimran, Gil, Shpilman, Michal, Mosquna, Assaf
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442787/ https://www.ncbi.nlm.nih.gov/pubmed/35895499 http://dx.doi.org/10.1021/acssynbio.2c00192

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