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Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia

PURPOSE: This study investigated the bacterial contamination level of the indoor air and surface of the operation room, surgical, and gynecology wards of Dilchora Referral hospitals between January and August 2020. METHODS: A laboratory based cross-sectional study was carried out on the OR and wards...

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Autores principales: Yimer, Robel Mekonnen, Alemu, Mesfin Kebede
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442909/
https://www.ncbi.nlm.nih.gov/pubmed/36071817
http://dx.doi.org/10.2147/IDR.S380774
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author Yimer, Robel Mekonnen
Alemu, Mesfin Kebede
author_facet Yimer, Robel Mekonnen
Alemu, Mesfin Kebede
author_sort Yimer, Robel Mekonnen
collection PubMed
description PURPOSE: This study investigated the bacterial contamination level of the indoor air and surface of the operation room, surgical, and gynecology wards of Dilchora Referral hospitals between January and August 2020. METHODS: A laboratory based cross-sectional study was carried out on the OR and wards of Dilchora referral hospital in Eastern Ethiopia. A passive air sampling method was used to collect 128 indoor air samples; the bacterial load was enumerated and the result was expressed as colony forming units (CFU/m(3)). Additional qualitative analysis was carried out to identify particular bacterial species that were isolated from the indoor air and swabs taken from the surface of the equipment using conventional techniques. All laboratory data were entered and analyzed using MS Excel 2007 and SPSS version 20. RESULTS: The mean bacterial counts of 94.63 CFU/dm/hr in major OR during active time as well as 509.75 and 509.38 CFU/dm/hr in male and female clothing rooms during the afternoon were unacceptable (>450 CFU/dm(2)). Similarly, 43.75% of the bacterial counts found in the afternoon samples fell short of Fisher’s criterion. The difference between the bacterial counts recorded in the morning and afternoon was significant (p=0.000). A total of 54 (42.2%) indoor air samples and 28 (93.3%) cotton swabs were positive for bacterial growth, with S. aureus (51.04%) and Bacillus sp (55%) being the dominant bacteria isolated from indoor air and the surface of equipment, respectively. CONCLUSION: The bacterial load of investigated wards is considerably “high” to “very high”, which implies a significant risk of hospital acquired infections. Therefore, devising effective control strategies targeted on surface cleansing and sterilizing of the air environment and practicing periodic microbial surveillance of the hospital environment is a paramant.
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spelling pubmed-94429092022-09-06 Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia Yimer, Robel Mekonnen Alemu, Mesfin Kebede Infect Drug Resist Original Research PURPOSE: This study investigated the bacterial contamination level of the indoor air and surface of the operation room, surgical, and gynecology wards of Dilchora Referral hospitals between January and August 2020. METHODS: A laboratory based cross-sectional study was carried out on the OR and wards of Dilchora referral hospital in Eastern Ethiopia. A passive air sampling method was used to collect 128 indoor air samples; the bacterial load was enumerated and the result was expressed as colony forming units (CFU/m(3)). Additional qualitative analysis was carried out to identify particular bacterial species that were isolated from the indoor air and swabs taken from the surface of the equipment using conventional techniques. All laboratory data were entered and analyzed using MS Excel 2007 and SPSS version 20. RESULTS: The mean bacterial counts of 94.63 CFU/dm/hr in major OR during active time as well as 509.75 and 509.38 CFU/dm/hr in male and female clothing rooms during the afternoon were unacceptable (>450 CFU/dm(2)). Similarly, 43.75% of the bacterial counts found in the afternoon samples fell short of Fisher’s criterion. The difference between the bacterial counts recorded in the morning and afternoon was significant (p=0.000). A total of 54 (42.2%) indoor air samples and 28 (93.3%) cotton swabs were positive for bacterial growth, with S. aureus (51.04%) and Bacillus sp (55%) being the dominant bacteria isolated from indoor air and the surface of equipment, respectively. CONCLUSION: The bacterial load of investigated wards is considerably “high” to “very high”, which implies a significant risk of hospital acquired infections. Therefore, devising effective control strategies targeted on surface cleansing and sterilizing of the air environment and practicing periodic microbial surveillance of the hospital environment is a paramant. Dove 2022-08-31 /pmc/articles/PMC9442909/ /pubmed/36071817 http://dx.doi.org/10.2147/IDR.S380774 Text en © 2022 Yimer and Alemu. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yimer, Robel Mekonnen
Alemu, Mesfin Kebede
Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title_full Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title_fullStr Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title_full_unstemmed Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title_short Bacterial Contamination Level of Indoor Air and Surface of Equipment in the Operation Room in Dil-Chora Referral Hospital, Dire Dawa, Eastern Ethiopia
title_sort bacterial contamination level of indoor air and surface of equipment in the operation room in dil-chora referral hospital, dire dawa, eastern ethiopia
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442909/
https://www.ncbi.nlm.nih.gov/pubmed/36071817
http://dx.doi.org/10.2147/IDR.S380774
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