Cargando…

The effectiveness of eugenol against cisplatin-induced ototoxicity()

INTRODUCTION: Ototoxicity refers to cellular damage or function impairment developing in the inner ear in association with any therapeutic agent or chemical substance, and still represents the principal side-effect restricting the use of cisplatin. OBJECTIVE: The aim of this study was to perform a b...

Descripción completa

Detalles Bibliográficos
Autores principales: Sakat, Muhammed Sedat, Kilic, Korhan, Akdemir, Fazile Nur Ekinci, Yildirim, Serkan, Eser, Gizem, Kiziltunc, Ahmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443064/
https://www.ncbi.nlm.nih.gov/pubmed/30126770
http://dx.doi.org/10.1016/j.bjorl.2018.07.007
_version_ 1784782963125256192
author Sakat, Muhammed Sedat
Kilic, Korhan
Akdemir, Fazile Nur Ekinci
Yildirim, Serkan
Eser, Gizem
Kiziltunc, Ahmet
author_facet Sakat, Muhammed Sedat
Kilic, Korhan
Akdemir, Fazile Nur Ekinci
Yildirim, Serkan
Eser, Gizem
Kiziltunc, Ahmet
author_sort Sakat, Muhammed Sedat
collection PubMed
description INTRODUCTION: Ototoxicity refers to cellular damage or function impairment developing in the inner ear in association with any therapeutic agent or chemical substance, and still represents the principal side-effect restricting the use of cisplatin. OBJECTIVE: The aim of this study was to perform a biochemical, functional and histopathological investigation of the potential protective effect of eugenol against cisplatin-induced ototoxicity. METHODS: The study was performed with 24 female Sprague Dawley rats. Distortion product otoacoustic emissions tests were performed on all animals, which were randomized into four equal groups. A single intraperitoneal dose of 15 mg/kg cisplatin was administered to cisplatin group, while the eugenol group received 100 mg/kg eugenol intraperitoneal for five consecutive days. 100 mg/kg eugenol was administered to cisplatin + eugenol group for 5 days. On the third day, these rats were received a single dose of 15 mg/kg cisplatin. The control group was given 8 mL/kg/day intraperitoneal saline solution for five days. The distortion product otoacoustic emissions test was repeated 24 h after the final drug administration. All animals were sacrificed, and the cochleas were subsequently used for biochemical and histopathological examinations. RESULTS: Cisplatin caused oxidative stress in the cochlea, impaired the cochlear structure and significantly reduced signal noise ratio levels. Administration of eugenol together with cisplatin reversed these effects and provided functional, biochemical and histopathological protection. CONCLUSION: The study findings represent the first indication in the literature that eugenol may protect against ototoxicity by raising levels of antioxidant enzymes and lowering those of oxidant parameters.
format Online
Article
Text
id pubmed-9443064
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-94430642022-09-09 The effectiveness of eugenol against cisplatin-induced ototoxicity() Sakat, Muhammed Sedat Kilic, Korhan Akdemir, Fazile Nur Ekinci Yildirim, Serkan Eser, Gizem Kiziltunc, Ahmet Braz J Otorhinolaryngol Original Article INTRODUCTION: Ototoxicity refers to cellular damage or function impairment developing in the inner ear in association with any therapeutic agent or chemical substance, and still represents the principal side-effect restricting the use of cisplatin. OBJECTIVE: The aim of this study was to perform a biochemical, functional and histopathological investigation of the potential protective effect of eugenol against cisplatin-induced ototoxicity. METHODS: The study was performed with 24 female Sprague Dawley rats. Distortion product otoacoustic emissions tests were performed on all animals, which were randomized into four equal groups. A single intraperitoneal dose of 15 mg/kg cisplatin was administered to cisplatin group, while the eugenol group received 100 mg/kg eugenol intraperitoneal for five consecutive days. 100 mg/kg eugenol was administered to cisplatin + eugenol group for 5 days. On the third day, these rats were received a single dose of 15 mg/kg cisplatin. The control group was given 8 mL/kg/day intraperitoneal saline solution for five days. The distortion product otoacoustic emissions test was repeated 24 h after the final drug administration. All animals were sacrificed, and the cochleas were subsequently used for biochemical and histopathological examinations. RESULTS: Cisplatin caused oxidative stress in the cochlea, impaired the cochlear structure and significantly reduced signal noise ratio levels. Administration of eugenol together with cisplatin reversed these effects and provided functional, biochemical and histopathological protection. CONCLUSION: The study findings represent the first indication in the literature that eugenol may protect against ototoxicity by raising levels of antioxidant enzymes and lowering those of oxidant parameters. Elsevier 2018-08-07 /pmc/articles/PMC9443064/ /pubmed/30126770 http://dx.doi.org/10.1016/j.bjorl.2018.07.007 Text en © 2018 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Sakat, Muhammed Sedat
Kilic, Korhan
Akdemir, Fazile Nur Ekinci
Yildirim, Serkan
Eser, Gizem
Kiziltunc, Ahmet
The effectiveness of eugenol against cisplatin-induced ototoxicity()
title The effectiveness of eugenol against cisplatin-induced ototoxicity()
title_full The effectiveness of eugenol against cisplatin-induced ototoxicity()
title_fullStr The effectiveness of eugenol against cisplatin-induced ototoxicity()
title_full_unstemmed The effectiveness of eugenol against cisplatin-induced ototoxicity()
title_short The effectiveness of eugenol against cisplatin-induced ototoxicity()
title_sort effectiveness of eugenol against cisplatin-induced ototoxicity()
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443064/
https://www.ncbi.nlm.nih.gov/pubmed/30126770
http://dx.doi.org/10.1016/j.bjorl.2018.07.007
work_keys_str_mv AT sakatmuhammedsedat theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT kilickorhan theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT akdemirfazilenurekinci theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT yildirimserkan theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT esergizem theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT kiziltuncahmet theeffectivenessofeugenolagainstcisplatininducedototoxicity
AT sakatmuhammedsedat effectivenessofeugenolagainstcisplatininducedototoxicity
AT kilickorhan effectivenessofeugenolagainstcisplatininducedototoxicity
AT akdemirfazilenurekinci effectivenessofeugenolagainstcisplatininducedototoxicity
AT yildirimserkan effectivenessofeugenolagainstcisplatininducedototoxicity
AT esergizem effectivenessofeugenolagainstcisplatininducedototoxicity
AT kiziltuncahmet effectivenessofeugenolagainstcisplatininducedototoxicity