Cargando…
In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice
Dysregulation of G1 cyclins (cyclins D1 A and E) expression contributes to the loss of standard cell cycle control during tumorigenesis. This study aims to evaluate the inhibitory effect of G1 cyclins in nude mice. The human breast cancer MDA-MB-231 cells were subcutaneously transplanted into the su...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443516/ https://www.ncbi.nlm.nih.gov/pubmed/36072388 http://dx.doi.org/10.3389/fvets.2022.914311 |
_version_ | 1784783000670568448 |
---|---|
author | Mesbah, Gholamreza Namazi, Fatemeh T. Shamsabadi, Fatemeh Maleki, Zahra Nasirikenari, Mehrab Shahbazi, Majid |
author_facet | Mesbah, Gholamreza Namazi, Fatemeh T. Shamsabadi, Fatemeh Maleki, Zahra Nasirikenari, Mehrab Shahbazi, Majid |
author_sort | Mesbah, Gholamreza |
collection | PubMed |
description | Dysregulation of G1 cyclins (cyclins D1 A and E) expression contributes to the loss of standard cell cycle control during tumorigenesis. This study aims to evaluate the inhibitory effect of G1 cyclins in nude mice. The human breast cancer MDA-MB-231 cells were subcutaneously transplanted into the supra-femoral right side of female Balb/c-nude mice. The dual shRNA vector harboring G1 cyclins shRNAs (bipSUR) was intratumorally injected by the in vivo jetPEI transfection reagent for 2 weeks. We have evaluated tumor growth and tumor weight as parameters of tumor progression. Finally, necropsy, histopathological analysis, and immunodetection of G1 cyclins were assessed. Also, apoptosis induction in tumor tissues was evaluated by TUNEL assay. No toxicity and metastasis was observed in the tumor-bearing mice treated by the bipSUR. Tumor weight and volume were significantly lower in the bipSUR treated mice than untreated tumor-bearing mice and control. Histopathological observations revealed more apoptotic foci and lower mitotic cells in tumor sections in the treated mice than in control groups. A significant reduction of G1 cyclins at the protein level was indicated in the bipSUR treated mice than in other groups. Apoptosis in tumor tissues was remarkably induced in response to the bipSUR (42.53%). The bipSUR reduced the protein expression of G1 cyclins and exhibited an inhibitory effect on MDA-MB-231 xenograft mice through apoptosis induction. Further research is demanded to identify the protein partners of G1 cyclins involved in the cancer pathways. These may offer new insight into the biomedical function of G1 cyclins in breast cancer progression. |
format | Online Article Text |
id | pubmed-9443516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94435162022-09-06 In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice Mesbah, Gholamreza Namazi, Fatemeh T. Shamsabadi, Fatemeh Maleki, Zahra Nasirikenari, Mehrab Shahbazi, Majid Front Vet Sci Veterinary Science Dysregulation of G1 cyclins (cyclins D1 A and E) expression contributes to the loss of standard cell cycle control during tumorigenesis. This study aims to evaluate the inhibitory effect of G1 cyclins in nude mice. The human breast cancer MDA-MB-231 cells were subcutaneously transplanted into the supra-femoral right side of female Balb/c-nude mice. The dual shRNA vector harboring G1 cyclins shRNAs (bipSUR) was intratumorally injected by the in vivo jetPEI transfection reagent for 2 weeks. We have evaluated tumor growth and tumor weight as parameters of tumor progression. Finally, necropsy, histopathological analysis, and immunodetection of G1 cyclins were assessed. Also, apoptosis induction in tumor tissues was evaluated by TUNEL assay. No toxicity and metastasis was observed in the tumor-bearing mice treated by the bipSUR. Tumor weight and volume were significantly lower in the bipSUR treated mice than untreated tumor-bearing mice and control. Histopathological observations revealed more apoptotic foci and lower mitotic cells in tumor sections in the treated mice than in control groups. A significant reduction of G1 cyclins at the protein level was indicated in the bipSUR treated mice than in other groups. Apoptosis in tumor tissues was remarkably induced in response to the bipSUR (42.53%). The bipSUR reduced the protein expression of G1 cyclins and exhibited an inhibitory effect on MDA-MB-231 xenograft mice through apoptosis induction. Further research is demanded to identify the protein partners of G1 cyclins involved in the cancer pathways. These may offer new insight into the biomedical function of G1 cyclins in breast cancer progression. Frontiers Media S.A. 2022-08-22 /pmc/articles/PMC9443516/ /pubmed/36072388 http://dx.doi.org/10.3389/fvets.2022.914311 Text en Copyright © 2022 Mesbah, Namazi, T. Shamsabadi, Maleki, Nasirikenari and Shahbazi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Mesbah, Gholamreza Namazi, Fatemeh T. Shamsabadi, Fatemeh Maleki, Zahra Nasirikenari, Mehrab Shahbazi, Majid In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title | In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title_full | In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title_fullStr | In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title_full_unstemmed | In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title_short | In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
title_sort | in vivo assessment of simultaneous g1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443516/ https://www.ncbi.nlm.nih.gov/pubmed/36072388 http://dx.doi.org/10.3389/fvets.2022.914311 |
work_keys_str_mv | AT mesbahgholamreza invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice AT namazifatemeh invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice AT tshamsabadifatemeh invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice AT malekizahra invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice AT nasirikenarimehrab invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice AT shahbazimajid invivoassessmentofsimultaneousg1cyclinssilencingbyatumorspecificbidirectionalpromoteronthemammarytumorinnudemice |