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Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies

Temporal bones are valuable resources to study ear diseases. Although there are several methods for removing temporal bones from cadavers, such methods are not usually described in enough details in experimental research papers. OBJECTIVES: To describe a simple and rapid method for ear canal and tym...

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Autores principales: e Gurgel, João Daniel Caliman, Pereira, Celina Siqueira Barbosa, Fregnani, José Humberto Tavares Guerreiro, de Andrade Quintanilha Ribeiro, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443901/
https://www.ncbi.nlm.nih.gov/pubmed/22392236
http://dx.doi.org/10.1590/S1808-86942012000100006
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author e Gurgel, João Daniel Caliman
Pereira, Celina Siqueira Barbosa
Fregnani, José Humberto Tavares Guerreiro
de Andrade Quintanilha Ribeiro, Fernando
author_facet e Gurgel, João Daniel Caliman
Pereira, Celina Siqueira Barbosa
Fregnani, José Humberto Tavares Guerreiro
de Andrade Quintanilha Ribeiro, Fernando
author_sort e Gurgel, João Daniel Caliman
collection PubMed
description Temporal bones are valuable resources to study ear diseases. Although there are several methods for removing temporal bones from cadavers, such methods are not usually described in enough details in experimental research papers. OBJECTIVES: To describe a simple and rapid method for ear canal and tympanic membrane removal, and to evaluate its viability for histologic and immunohistochemical studies. MATERIALS AND METHODS: In this experimental study, we obtained 31 ear canal and tympanic membrane samples from cadavers, with a conventional power drill and plug cutter. The material was dissected and samples containing ear canals and tympanic membranes were obtained in blocks. The samples were analyzed by histology and immunohistochemistry. RESULTS: Removal of small and good quality samples containing entire ear canals and tympanic membranes. In all the samples, it was possible to perform both histological and immunohistochemical analyses. CONCLUSION: This method was easily achievable, reproducible and yielded good quality samples, both for training purposes and for experimental research. All the samples were viable for histological and immunohistochemical analyses.
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spelling pubmed-94439012022-09-09 Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies e Gurgel, João Daniel Caliman Pereira, Celina Siqueira Barbosa Fregnani, José Humberto Tavares Guerreiro de Andrade Quintanilha Ribeiro, Fernando Braz J Otorhinolaryngol Original Article Temporal bones are valuable resources to study ear diseases. Although there are several methods for removing temporal bones from cadavers, such methods are not usually described in enough details in experimental research papers. OBJECTIVES: To describe a simple and rapid method for ear canal and tympanic membrane removal, and to evaluate its viability for histologic and immunohistochemical studies. MATERIALS AND METHODS: In this experimental study, we obtained 31 ear canal and tympanic membrane samples from cadavers, with a conventional power drill and plug cutter. The material was dissected and samples containing ear canals and tympanic membranes were obtained in blocks. The samples were analyzed by histology and immunohistochemistry. RESULTS: Removal of small and good quality samples containing entire ear canals and tympanic membranes. In all the samples, it was possible to perform both histological and immunohistochemical analyses. CONCLUSION: This method was easily achievable, reproducible and yielded good quality samples, both for training purposes and for experimental research. All the samples were viable for histological and immunohistochemical analyses. Elsevier 2015-10-20 /pmc/articles/PMC9443901/ /pubmed/22392236 http://dx.doi.org/10.1590/S1808-86942012000100006 Text en . https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
e Gurgel, João Daniel Caliman
Pereira, Celina Siqueira Barbosa
Fregnani, José Humberto Tavares Guerreiro
de Andrade Quintanilha Ribeiro, Fernando
Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title_full Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title_fullStr Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title_full_unstemmed Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title_short Method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
title_sort method for removal of samples from the external ear canal and the tympanic membrane for histological and immunohistochemical studies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443901/
https://www.ncbi.nlm.nih.gov/pubmed/22392236
http://dx.doi.org/10.1590/S1808-86942012000100006
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