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Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis

Alternative splicing (AS) is a process by which several functional splice variants are generated from the same precursor mRNA. In our recent study, five CsA-IPT5 splice variants with various numbers of ATTTA motifs in the untranslated regions (UTRs) were cloned. Meanwhile, their transient expression...

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Autores principales: Zhang, Liping, Wang, Donghui, Zhang, Lan, Fu, Jianyu, Yan, Peng, Ge, Shibei, Li, Zhengzhen, Ahammed, Golam Jalal, Han, Wenyan, Li, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444062/
https://www.ncbi.nlm.nih.gov/pubmed/36072311
http://dx.doi.org/10.3389/fpls.2022.977086
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author Zhang, Liping
Wang, Donghui
Zhang, Lan
Fu, Jianyu
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Ahammed, Golam Jalal
Han, Wenyan
Li, Xin
author_facet Zhang, Liping
Wang, Donghui
Zhang, Lan
Fu, Jianyu
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Ahammed, Golam Jalal
Han, Wenyan
Li, Xin
author_sort Zhang, Liping
collection PubMed
description Alternative splicing (AS) is a process by which several functional splice variants are generated from the same precursor mRNA. In our recent study, five CsA-IPT5 splice variants with various numbers of ATTTA motifs in the untranslated regions (UTRs) were cloned. Meanwhile, their transient expression, as well as the expression and functional analysis in the two shoot branching processes were studied. Here, we examined how these splice variants regulate the other three important shoot branching processes, including the spring tea development, the distal branching of new shoots, and the shoot branching induced by 2,3,5-triiodobenzoic acid (TIBA) spraying, and thus unraveling the key CsA-IPT5 transcripts which play the most important roles in the shoot branching of tea plants. The results showed that the increased expression of 5′ UTR AS3, 3′ UTR AS1 and 3′ UTR AS2 could contribute to the increased synthesis of tZ/iP-type cytokinins (CKs), thus promoting the spring tea development. Meanwhile, in the TIBA-induced shoot branching or in the distal branching of the new shoots, CsA-IPT5 transcripts regulated the synthesis of CsA-IPT5 protein and CKs through transcriptional regulation of the ratios of its splice variants. Moreover, 3′ UTR AS1 and 3′ UTR AS2 both play key roles in these two processes. In summary, it is revealed that 3′ UTR AS1 and 3′ UTR AS2 of CsA-IPT5 might act as the predominant splice variants in shoot branching of the tea plant, and they both can serve as gene resources for tea plant breeding.
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spelling pubmed-94440622022-09-06 Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis Zhang, Liping Wang, Donghui Zhang, Lan Fu, Jianyu Yan, Peng Ge, Shibei Li, Zhengzhen Ahammed, Golam Jalal Han, Wenyan Li, Xin Front Plant Sci Plant Science Alternative splicing (AS) is a process by which several functional splice variants are generated from the same precursor mRNA. In our recent study, five CsA-IPT5 splice variants with various numbers of ATTTA motifs in the untranslated regions (UTRs) were cloned. Meanwhile, their transient expression, as well as the expression and functional analysis in the two shoot branching processes were studied. Here, we examined how these splice variants regulate the other three important shoot branching processes, including the spring tea development, the distal branching of new shoots, and the shoot branching induced by 2,3,5-triiodobenzoic acid (TIBA) spraying, and thus unraveling the key CsA-IPT5 transcripts which play the most important roles in the shoot branching of tea plants. The results showed that the increased expression of 5′ UTR AS3, 3′ UTR AS1 and 3′ UTR AS2 could contribute to the increased synthesis of tZ/iP-type cytokinins (CKs), thus promoting the spring tea development. Meanwhile, in the TIBA-induced shoot branching or in the distal branching of the new shoots, CsA-IPT5 transcripts regulated the synthesis of CsA-IPT5 protein and CKs through transcriptional regulation of the ratios of its splice variants. Moreover, 3′ UTR AS1 and 3′ UTR AS2 both play key roles in these two processes. In summary, it is revealed that 3′ UTR AS1 and 3′ UTR AS2 of CsA-IPT5 might act as the predominant splice variants in shoot branching of the tea plant, and they both can serve as gene resources for tea plant breeding. Frontiers Media S.A. 2022-08-22 /pmc/articles/PMC9444062/ /pubmed/36072311 http://dx.doi.org/10.3389/fpls.2022.977086 Text en Copyright © 2022 Zhang, Wang, Zhang, Fu, Yan, Ge, Li, Ahammed, Han and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Liping
Wang, Donghui
Zhang, Lan
Fu, Jianyu
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Ahammed, Golam Jalal
Han, Wenyan
Li, Xin
Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title_full Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title_fullStr Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title_full_unstemmed Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title_short Expression and functional analysis of CsA-IPT5 splice variants during shoot branching in Camellia sinensis
title_sort expression and functional analysis of csa-ipt5 splice variants during shoot branching in camellia sinensis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444062/
https://www.ncbi.nlm.nih.gov/pubmed/36072311
http://dx.doi.org/10.3389/fpls.2022.977086
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