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New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear mar...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444205/ https://www.ncbi.nlm.nih.gov/pubmed/36118371 http://dx.doi.org/10.2478/helm-2022-0021 |
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author | Chaouadi, M. Scarpa, F. Azzena, I. Cossu, P. Harhoura, K. Aissi, M. Tazerouti, F. Garippa, G. Merella, P. Casu, M. Sanna, D. |
author_facet | Chaouadi, M. Scarpa, F. Azzena, I. Cossu, P. Harhoura, K. Aissi, M. Tazerouti, F. Garippa, G. Merella, P. Casu, M. Sanna, D. |
author_sort | Chaouadi, M. |
collection | PubMed |
description | This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution. |
format | Online Article Text |
id | pubmed-9444205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-94442052022-09-16 New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA Chaouadi, M. Scarpa, F. Azzena, I. Cossu, P. Harhoura, K. Aissi, M. Tazerouti, F. Garippa, G. Merella, P. Casu, M. Sanna, D. Helminthologia Research Article This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution. Sciendo 2022-09-03 /pmc/articles/PMC9444205/ /pubmed/36118371 http://dx.doi.org/10.2478/helm-2022-0021 Text en © 2022 M. Chaouadi, F. Scarpa, I. Azzena, P. Cossu, K. Harhoura, M. Aissi, F. Tazerouti, G. Garippa, P. Merella, M. Casu, D. Sanna, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Research Article Chaouadi, M. Scarpa, F. Azzena, I. Cossu, P. Harhoura, K. Aissi, M. Tazerouti, F. Garippa, G. Merella, P. Casu, M. Sanna, D. New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title | New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title_full | New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title_fullStr | New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title_full_unstemmed | New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title_short | New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA |
title_sort | new insights into the genetic variability of fasciola hepatica (trematoda) in algeria and relationships with other geographic regions revealed by mitochondrial dna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444205/ https://www.ncbi.nlm.nih.gov/pubmed/36118371 http://dx.doi.org/10.2478/helm-2022-0021 |
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