Cargando…

New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA

This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear mar...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaouadi, M., Scarpa, F., Azzena, I., Cossu, P., Harhoura, K., Aissi, M., Tazerouti, F., Garippa, G., Merella, P., Casu, M., Sanna, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444205/
https://www.ncbi.nlm.nih.gov/pubmed/36118371
http://dx.doi.org/10.2478/helm-2022-0021
_version_ 1784783165780393984
author Chaouadi, M.
Scarpa, F.
Azzena, I.
Cossu, P.
Harhoura, K.
Aissi, M.
Tazerouti, F.
Garippa, G.
Merella, P.
Casu, M.
Sanna, D.
author_facet Chaouadi, M.
Scarpa, F.
Azzena, I.
Cossu, P.
Harhoura, K.
Aissi, M.
Tazerouti, F.
Garippa, G.
Merella, P.
Casu, M.
Sanna, D.
author_sort Chaouadi, M.
collection PubMed
description This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.
format Online
Article
Text
id pubmed-9444205
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-94442052022-09-16 New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA Chaouadi, M. Scarpa, F. Azzena, I. Cossu, P. Harhoura, K. Aissi, M. Tazerouti, F. Garippa, G. Merella, P. Casu, M. Sanna, D. Helminthologia Research Article This study aims to investigate the level of genetic variability of Fasciola hepatica flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 F. hepatica flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among F. hepatica isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of F. hepatica are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution. Sciendo 2022-09-03 /pmc/articles/PMC9444205/ /pubmed/36118371 http://dx.doi.org/10.2478/helm-2022-0021 Text en © 2022 M. Chaouadi, F. Scarpa, I. Azzena, P. Cossu, K. Harhoura, M. Aissi, F. Tazerouti, G. Garippa, P. Merella, M. Casu, D. Sanna, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Research Article
Chaouadi, M.
Scarpa, F.
Azzena, I.
Cossu, P.
Harhoura, K.
Aissi, M.
Tazerouti, F.
Garippa, G.
Merella, P.
Casu, M.
Sanna, D.
New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title_full New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title_fullStr New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title_full_unstemmed New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title_short New Insights Into the Genetic Variability of Fasciola Hepatica (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA
title_sort new insights into the genetic variability of fasciola hepatica (trematoda) in algeria and relationships with other geographic regions revealed by mitochondrial dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444205/
https://www.ncbi.nlm.nih.gov/pubmed/36118371
http://dx.doi.org/10.2478/helm-2022-0021
work_keys_str_mv AT chaouadim newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT scarpaf newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT azzenai newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT cossup newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT harhourak newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT aissim newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT tazeroutif newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT garippag newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT merellap newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT casum newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna
AT sannad newinsightsintothegeneticvariabilityoffasciolahepaticatrematodainalgeriaandrelationshipswithothergeographicregionsrevealedbymitochondrialdna