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CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry

The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell l...

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Detalles Bibliográficos
Autores principales: Heinl, Elena-Sofia, Lorenz, Sebastian, Schmidt, Barbara, Nasser M Laqtom, Nouf, Mazzulli, Joseph R., Francelle, Laetitia, Yu, Timothy W., Greenberg, Benjamin, Storch, Stephan, Tegtmeier, Ines, Othmen, Helga, Maurer, Katja, Steinfurth, Malin, Witzgall, Ralph, Milenkovic, Vladimir, Wetzel, Christian H., Reichold, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444308/
https://www.ncbi.nlm.nih.gov/pubmed/36093380
http://dx.doi.org/10.1016/j.isci.2022.105082
Descripción
Sumario:The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line exhibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evidence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.