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Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()

INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes invol...

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Autores principales: Barbosa, Andressa, dos Santos, Marcelo, de Podestá, José Roberto Vasconcelos, Gouvêa, Sônia Alves, Von Zeidler, Sandra Ventorin, Louro, Iúri Drumond, Cordeiro-Silva, Melissa de Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444640/
https://www.ncbi.nlm.nih.gov/pubmed/26749456
http://dx.doi.org/10.1016/j.bjorl.2015.10.012
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author Barbosa, Andressa
dos Santos, Marcelo
de Podestá, José Roberto Vasconcelos
Gouvêa, Sônia Alves
Von Zeidler, Sandra Ventorin
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
author_facet Barbosa, Andressa
dos Santos, Marcelo
de Podestá, José Roberto Vasconcelos
Gouvêa, Sônia Alves
Von Zeidler, Sandra Ventorin
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
author_sort Barbosa, Andressa
collection PubMed
description INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. OBJECTIVE: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. METHODS: This case–control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis. RESULTS: MTHFR C677T polymorphism was associated with lymph node involvement. Genotype CT + TT acted as a protective factor. MTHFR A1298C AC + CC genotype was associated with tumor differentiation, and possibly with a better prognosis. In risk analysis, no correlation was observed between genotypes and OSCC. CONCLUSION: We concluded that MTHFR C677T, MTHFR A1298C and CBS 844ins68 polymorphisms were not associated with OSCC risk in southeastern Brazilians; however, we suggest a prognosis effect associated with MTHFR C677T and A1298C polymorphisms in OSCC.
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spelling pubmed-94446402022-09-09 Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians() Barbosa, Andressa dos Santos, Marcelo de Podestá, José Roberto Vasconcelos Gouvêa, Sônia Alves Von Zeidler, Sandra Ventorin Louro, Iúri Drumond Cordeiro-Silva, Melissa de Freitas Braz J Otorhinolaryngol Original Article INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. OBJECTIVE: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. METHODS: This case–control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis. RESULTS: MTHFR C677T polymorphism was associated with lymph node involvement. Genotype CT + TT acted as a protective factor. MTHFR A1298C AC + CC genotype was associated with tumor differentiation, and possibly with a better prognosis. In risk analysis, no correlation was observed between genotypes and OSCC. CONCLUSION: We concluded that MTHFR C677T, MTHFR A1298C and CBS 844ins68 polymorphisms were not associated with OSCC risk in southeastern Brazilians; however, we suggest a prognosis effect associated with MTHFR C677T and A1298C polymorphisms in OSCC. Elsevier 2015-12-17 /pmc/articles/PMC9444640/ /pubmed/26749456 http://dx.doi.org/10.1016/j.bjorl.2015.10.012 Text en © 2015 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Barbosa, Andressa
dos Santos, Marcelo
de Podestá, José Roberto Vasconcelos
Gouvêa, Sônia Alves
Von Zeidler, Sandra Ventorin
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title_full Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title_fullStr Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title_full_unstemmed Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title_short Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern Brazilians()
title_sort polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer – a case–control study in southeastern brazilians()
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444640/
https://www.ncbi.nlm.nih.gov/pubmed/26749456
http://dx.doi.org/10.1016/j.bjorl.2015.10.012
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