Cargando…

Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers

This study aimed to investigate the effect of chronic heat stress on oxidative stress in liver of broilers. In our study, chickens were randomly allocated to control 1 group (control 7 d), heat stress 1 group (HS1, 7 d), control 2 group (control 14 d) and heat stress 2 group (HS2, 14 d), with 30 rep...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Lu-Ping, Liu, Yi-Lei, Zhang, Jia-Xin, Ding, Kang-Ning, Lu, Meng-Han, He, Yong-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445375/
https://www.ncbi.nlm.nih.gov/pubmed/36055022
http://dx.doi.org/10.1016/j.psj.2022.102085
_version_ 1784783407644934144
author Tang, Lu-Ping
Liu, Yi-Lei
Zhang, Jia-Xin
Ding, Kang-Ning
Lu, Meng-Han
He, Yong-Ming
author_facet Tang, Lu-Ping
Liu, Yi-Lei
Zhang, Jia-Xin
Ding, Kang-Ning
Lu, Meng-Han
He, Yong-Ming
author_sort Tang, Lu-Ping
collection PubMed
description This study aimed to investigate the effect of chronic heat stress on oxidative stress in liver of broilers. In our study, chickens were randomly allocated to control 1 group (control 7 d), heat stress 1 group (HS1, 7 d), control 2 group (control 14 d) and heat stress 2 group (HS2, 14 d), with 30 replicates in each group. Broilers in heat stress groups exposed 8 h/day heat stress (35 ± 2°C) for 7 or 14 consecutive days, and the rest of time per day were kept at 23 ± 2℃ the same as control group broilers. Growth performance and the liver tissues were collected for histological observation and detection of organ index and liver redox status. The serum indicators (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]) related to liver injury were determined. Moreover, Nrf2-related genes and protein expression levels in liver were measured. The results showed that in heat stress group broilers the body weight gain, feed conversion ratio, liver weight, and liver index were decreased, inflammatory cells infiltration in liver, and serum AST level was enhanced, compared with control group broilers. Moreover, the hepatic malondialdehyde (MDA) and superoxide dismutase (SOD) level were increased after 1 wk of heat stress. Nrf2, Sqstm1/p62, HO-1, and NQO1 mRNA expressions in the liver of broilers were decreased by heat stress. P62 and p-p62 protein expressions were significantly up-regulated, but Nrf2 and keap1 protein level was decreased in heat stress group broilers as compared to control group. The mRNA expression levels of Beclin1, LC3-I, LC3-II were down-regulated significantly with heat stress for 1 wk. The mRNA expression level of mTOR up-regulated after 2 wk of heat stress. In conclusion, heat stress induced liver injury of broilers by down-regulating Nrf2-keap1 signaling pathway and autophagy.
format Online
Article
Text
id pubmed-9445375
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-94453752022-09-07 Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers Tang, Lu-Ping Liu, Yi-Lei Zhang, Jia-Xin Ding, Kang-Ning Lu, Meng-Han He, Yong-Ming Poult Sci IMMUNOLOGY, HEALTH AND DISEASE This study aimed to investigate the effect of chronic heat stress on oxidative stress in liver of broilers. In our study, chickens were randomly allocated to control 1 group (control 7 d), heat stress 1 group (HS1, 7 d), control 2 group (control 14 d) and heat stress 2 group (HS2, 14 d), with 30 replicates in each group. Broilers in heat stress groups exposed 8 h/day heat stress (35 ± 2°C) for 7 or 14 consecutive days, and the rest of time per day were kept at 23 ± 2℃ the same as control group broilers. Growth performance and the liver tissues were collected for histological observation and detection of organ index and liver redox status. The serum indicators (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]) related to liver injury were determined. Moreover, Nrf2-related genes and protein expression levels in liver were measured. The results showed that in heat stress group broilers the body weight gain, feed conversion ratio, liver weight, and liver index were decreased, inflammatory cells infiltration in liver, and serum AST level was enhanced, compared with control group broilers. Moreover, the hepatic malondialdehyde (MDA) and superoxide dismutase (SOD) level were increased after 1 wk of heat stress. Nrf2, Sqstm1/p62, HO-1, and NQO1 mRNA expressions in the liver of broilers were decreased by heat stress. P62 and p-p62 protein expressions were significantly up-regulated, but Nrf2 and keap1 protein level was decreased in heat stress group broilers as compared to control group. The mRNA expression levels of Beclin1, LC3-I, LC3-II were down-regulated significantly with heat stress for 1 wk. The mRNA expression level of mTOR up-regulated after 2 wk of heat stress. In conclusion, heat stress induced liver injury of broilers by down-regulating Nrf2-keap1 signaling pathway and autophagy. Elsevier 2022-08-01 /pmc/articles/PMC9445375/ /pubmed/36055022 http://dx.doi.org/10.1016/j.psj.2022.102085 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Tang, Lu-Ping
Liu, Yi-Lei
Zhang, Jia-Xin
Ding, Kang-Ning
Lu, Meng-Han
He, Yong-Ming
Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title_full Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title_fullStr Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title_full_unstemmed Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title_short Heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
title_sort heat stress in broilers of liver injury effects of heat stress on oxidative stress and autophagy in liver of broilers
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445375/
https://www.ncbi.nlm.nih.gov/pubmed/36055022
http://dx.doi.org/10.1016/j.psj.2022.102085
work_keys_str_mv AT tangluping heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers
AT liuyilei heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers
AT zhangjiaxin heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers
AT dingkangning heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers
AT lumenghan heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers
AT heyongming heatstressinbroilersofliverinjuryeffectsofheatstressonoxidativestressandautophagyinliverofbroilers