Optimization of the CYP inhibition assay using LC-MS/MS

The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bio...

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Autores principales: Abduraman, Muhammad Asyraf, Mustafa, Nor Hidayah, Yaacob, Nik Soriani, Amanah, Azimah, Tan, Mei Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445379/
https://www.ncbi.nlm.nih.gov/pubmed/36081487
http://dx.doi.org/10.1016/j.mex.2022.101827
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author Abduraman, Muhammad Asyraf
Mustafa, Nor Hidayah
Yaacob, Nik Soriani
Amanah, Azimah
Tan, Mei Lan
author_facet Abduraman, Muhammad Asyraf
Mustafa, Nor Hidayah
Yaacob, Nik Soriani
Amanah, Azimah
Tan, Mei Lan
author_sort Abduraman, Muhammad Asyraf
collection PubMed
description The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1’-hydroxylase assay using LC-MS/MS.
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spelling pubmed-94453792022-09-07 Optimization of the CYP inhibition assay using LC-MS/MS Abduraman, Muhammad Asyraf Mustafa, Nor Hidayah Yaacob, Nik Soriani Amanah, Azimah Tan, Mei Lan MethodsX Protocol Article The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1’-hydroxylase assay using LC-MS/MS. Elsevier 2022-08-23 /pmc/articles/PMC9445379/ /pubmed/36081487 http://dx.doi.org/10.1016/j.mex.2022.101827 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol Article
Abduraman, Muhammad Asyraf
Mustafa, Nor Hidayah
Yaacob, Nik Soriani
Amanah, Azimah
Tan, Mei Lan
Optimization of the CYP inhibition assay using LC-MS/MS
title Optimization of the CYP inhibition assay using LC-MS/MS
title_full Optimization of the CYP inhibition assay using LC-MS/MS
title_fullStr Optimization of the CYP inhibition assay using LC-MS/MS
title_full_unstemmed Optimization of the CYP inhibition assay using LC-MS/MS
title_short Optimization of the CYP inhibition assay using LC-MS/MS
title_sort optimization of the cyp inhibition assay using lc-ms/ms
topic Protocol Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445379/
https://www.ncbi.nlm.nih.gov/pubmed/36081487
http://dx.doi.org/10.1016/j.mex.2022.101827
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AT amanahazimah optimizationofthecypinhibitionassayusinglcmsms
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