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Mini Bioreactor Can Support In Vitro Spermatogenesis of Mouse Testicular Tissue
OBJECTIVE: It was in the early 20(th) century when the quest for in vitro spermatogenesis started. In vitro spermatogenesis is critical for male cancer patients undergoing gonadotoxic treatment. Dynamic culture system creates in vivo-like conditions. In this study, it was intended to evaluate the pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445517/ https://www.ncbi.nlm.nih.gov/pubmed/35717571 http://dx.doi.org/10.22074/cellj.2022.8053 |
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author | Amirkhani, Zahra Movahedin, Mansoureh Baheiraei, Nafiseh Ghiaseddin, Ali |
author_facet | Amirkhani, Zahra Movahedin, Mansoureh Baheiraei, Nafiseh Ghiaseddin, Ali |
author_sort | Amirkhani, Zahra |
collection | PubMed |
description | OBJECTIVE: It was in the early 20(th) century when the quest for in vitro spermatogenesis started. In vitro spermatogenesis is critical for male cancer patients undergoing gonadotoxic treatment. Dynamic culture system creates in vivo-like conditions. In this study, it was intended to evaluate the progression of spermatogenesis after testicular tissue culture in mini-perfusion bioreactor. MATERIALS AND METHODS: In this experimental study, 12 six-day postpartum neonatal mouse testeswere removed and fragmented, placed on an agarose gel in parallel to bioreactor culture, and incubated for 8 weeks. Histological, molecular and immunohistochemical evaluations were carried out after 8 weeks. RESULTS: Histological analysis suggested successful maintenance of spermatogenesis in tissues grown in the bioreactor but not on agarose gel, possibly because the central region did not receive sufficient oxygen and nutrients, which led to necrotic or degenerative changes. Molecular analysis indicated that Plzf, Tekt1 and Tnp1 were expressed and that their expression did not differ significantly between the bioreactor and agarose gel. Immunohistochemical evaluation of testis fragments showed that PLZF, SCP3 and ACRBP proteins were expressed in spermatogonial cells, spermatocytes and spermatozoa. PLZF expression after 8 weeks was significantly lower (P<0.05) in tissues incubated on agarose gel than in the bioreactor, but there was no significant difference between SCP3 and ACRBP expression among the bioreactor and agarose gel culture systems. CONCLUSION: This three-dimensional (3D) dynamic culture system can provide somewhat similar conditions to the physiological environment of the testis. Our findings suggest that the perfusion bioreactor supports induction of spermatogenesis for generation of haploid cells. Further studies will be needed to address the fertility of the sperm generated in the bioreactor system. |
format | Online Article Text |
id | pubmed-9445517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-94455172022-09-23 Mini Bioreactor Can Support In Vitro Spermatogenesis of Mouse Testicular Tissue Amirkhani, Zahra Movahedin, Mansoureh Baheiraei, Nafiseh Ghiaseddin, Ali Cell J Original Article OBJECTIVE: It was in the early 20(th) century when the quest for in vitro spermatogenesis started. In vitro spermatogenesis is critical for male cancer patients undergoing gonadotoxic treatment. Dynamic culture system creates in vivo-like conditions. In this study, it was intended to evaluate the progression of spermatogenesis after testicular tissue culture in mini-perfusion bioreactor. MATERIALS AND METHODS: In this experimental study, 12 six-day postpartum neonatal mouse testeswere removed and fragmented, placed on an agarose gel in parallel to bioreactor culture, and incubated for 8 weeks. Histological, molecular and immunohistochemical evaluations were carried out after 8 weeks. RESULTS: Histological analysis suggested successful maintenance of spermatogenesis in tissues grown in the bioreactor but not on agarose gel, possibly because the central region did not receive sufficient oxygen and nutrients, which led to necrotic or degenerative changes. Molecular analysis indicated that Plzf, Tekt1 and Tnp1 were expressed and that their expression did not differ significantly between the bioreactor and agarose gel. Immunohistochemical evaluation of testis fragments showed that PLZF, SCP3 and ACRBP proteins were expressed in spermatogonial cells, spermatocytes and spermatozoa. PLZF expression after 8 weeks was significantly lower (P<0.05) in tissues incubated on agarose gel than in the bioreactor, but there was no significant difference between SCP3 and ACRBP expression among the bioreactor and agarose gel culture systems. CONCLUSION: This three-dimensional (3D) dynamic culture system can provide somewhat similar conditions to the physiological environment of the testis. Our findings suggest that the perfusion bioreactor supports induction of spermatogenesis for generation of haploid cells. Further studies will be needed to address the fertility of the sperm generated in the bioreactor system. Royan Institute 2022-05 2022-05-28 /pmc/articles/PMC9445517/ /pubmed/35717571 http://dx.doi.org/10.22074/cellj.2022.8053 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Amirkhani, Zahra Movahedin, Mansoureh Baheiraei, Nafiseh Ghiaseddin, Ali Mini Bioreactor Can Support In Vitro Spermatogenesis of Mouse Testicular Tissue |
title | Mini Bioreactor Can Support In Vitro Spermatogenesis of
Mouse Testicular Tissue |
title_full | Mini Bioreactor Can Support In Vitro Spermatogenesis of
Mouse Testicular Tissue |
title_fullStr | Mini Bioreactor Can Support In Vitro Spermatogenesis of
Mouse Testicular Tissue |
title_full_unstemmed | Mini Bioreactor Can Support In Vitro Spermatogenesis of
Mouse Testicular Tissue |
title_short | Mini Bioreactor Can Support In Vitro Spermatogenesis of
Mouse Testicular Tissue |
title_sort | mini bioreactor can support in vitro spermatogenesis of
mouse testicular tissue |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445517/ https://www.ncbi.nlm.nih.gov/pubmed/35717571 http://dx.doi.org/10.22074/cellj.2022.8053 |
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