Cargando…

Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway

OBJECTIVE: Cordycepin, also known as 3ˊ-deoxyadenosine, is the main bioactive ingredient of Cordyceps militaris and possesses various pharmacological effects. This study was performed to investigate the role of cordycepin in regulating the biological behaviors of colon cancer cells and the potential...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Jie, Shen, Xia, Sun, Daozhong, Zhu, Yanjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445518/
https://www.ncbi.nlm.nih.gov/pubmed/35717567
http://dx.doi.org/10.22074/cellj.2022.8160
_version_ 1784783440943513600
author Xu, Jie
Shen, Xia
Sun, Daozhong
Zhu, Yanjie
author_facet Xu, Jie
Shen, Xia
Sun, Daozhong
Zhu, Yanjie
author_sort Xu, Jie
collection PubMed
description OBJECTIVE: Cordycepin, also known as 3ˊ-deoxyadenosine, is the main bioactive ingredient of Cordyceps militaris and possesses various pharmacological effects. This study was performed to investigate the role of cordycepin in regulating the biological behaviors of colon cancer cells and the potential mechanism behind it. MATERIALS AND METHODS: In this experimental study, after treatment of colon cancer cells with different concentrations of cordycepin, inhibition of proliferation was detected by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Colon cancer cell migration and invasion abilities were analyzed by wound healing and Transwell assays. Flow cytometry was performed to detect cell apoptosis. A lung metastasis model in nude mice was utilized to examine the effect of cordycepin on the metastasis of colon cancer cells in vivo. Western blot was used to quantify GSK3β, β-catenin and cyclin D1 expression levels. RESULTS: Cordycepin inhibited colon cancer cell proliferation, migration and invasion, induced apoptosis in vitro, and inhibited lung metastasis of colon cancer cells in vivo. GSK-3β inhibitor (CHIR99021) treatment abolished the effects of cordycepin on cell viability, migration, invasion and apoptosis. Additionally, cordycepin promoted the expressions of GSK3β, and inhibited β-catenin and cyclin D1 in colon cancer cells, while co-treatment with CHIR99021 reversed the above effects. CONCLUSION: Cordycepin suppresses the malignant phenotypes of colon cancer through the GSK3β/β-catenin/cyclin D1 signaling pathway.
format Online
Article
Text
id pubmed-9445518
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Royan Institute
record_format MEDLINE/PubMed
spelling pubmed-94455182022-09-23 Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway Xu, Jie Shen, Xia Sun, Daozhong Zhu, Yanjie Cell J Original Article OBJECTIVE: Cordycepin, also known as 3ˊ-deoxyadenosine, is the main bioactive ingredient of Cordyceps militaris and possesses various pharmacological effects. This study was performed to investigate the role of cordycepin in regulating the biological behaviors of colon cancer cells and the potential mechanism behind it. MATERIALS AND METHODS: In this experimental study, after treatment of colon cancer cells with different concentrations of cordycepin, inhibition of proliferation was detected by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Colon cancer cell migration and invasion abilities were analyzed by wound healing and Transwell assays. Flow cytometry was performed to detect cell apoptosis. A lung metastasis model in nude mice was utilized to examine the effect of cordycepin on the metastasis of colon cancer cells in vivo. Western blot was used to quantify GSK3β, β-catenin and cyclin D1 expression levels. RESULTS: Cordycepin inhibited colon cancer cell proliferation, migration and invasion, induced apoptosis in vitro, and inhibited lung metastasis of colon cancer cells in vivo. GSK-3β inhibitor (CHIR99021) treatment abolished the effects of cordycepin on cell viability, migration, invasion and apoptosis. Additionally, cordycepin promoted the expressions of GSK3β, and inhibited β-catenin and cyclin D1 in colon cancer cells, while co-treatment with CHIR99021 reversed the above effects. CONCLUSION: Cordycepin suppresses the malignant phenotypes of colon cancer through the GSK3β/β-catenin/cyclin D1 signaling pathway. Royan Institute 2022-05 2022-05-28 /pmc/articles/PMC9445518/ /pubmed/35717567 http://dx.doi.org/10.22074/cellj.2022.8160 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Xu, Jie
Shen, Xia
Sun, Daozhong
Zhu, Yanjie
Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title_full Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title_fullStr Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title_full_unstemmed Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title_short Cordycepin Suppresses The Malignant Phenotypes of Colon Cancer Cells through The GSK3ß/ß-catenin/cyclin D1 Signaling Pathway
title_sort cordycepin suppresses the malignant phenotypes of colon cancer cells through the gsk3ß/ß-catenin/cyclin d1 signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445518/
https://www.ncbi.nlm.nih.gov/pubmed/35717567
http://dx.doi.org/10.22074/cellj.2022.8160
work_keys_str_mv AT xujie cordycepinsuppressesthemalignantphenotypesofcoloncancercellsthroughthegsk3ßßcatenincyclind1signalingpathway
AT shenxia cordycepinsuppressesthemalignantphenotypesofcoloncancercellsthroughthegsk3ßßcatenincyclind1signalingpathway
AT sundaozhong cordycepinsuppressesthemalignantphenotypesofcoloncancercellsthroughthegsk3ßßcatenincyclind1signalingpathway
AT zhuyanjie cordycepinsuppressesthemalignantphenotypesofcoloncancercellsthroughthegsk3ßßcatenincyclind1signalingpathway