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Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa

Growth-regulating factors (GRFs) play crucial roles in plant growth and stress response. To date, there have been no reports of the analysis and identification of the GRF transcription factor family in alfalfa. In this study, we identified 27 GRF family members from alfalfa (Medicago sativa L.) “Xin...

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Autores principales: Sun, Yue, Li, He, Wu, Jiajing, Zhang, Kangning, Tang, Wei, Cong, Lili, Xie, Hongli, Wang, Zeng-Yu, Chai, Maofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445573/
https://www.ncbi.nlm.nih.gov/pubmed/36082290
http://dx.doi.org/10.3389/fpls.2022.964604
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author Sun, Yue
Li, He
Wu, Jiajing
Zhang, Kangning
Tang, Wei
Cong, Lili
Xie, Hongli
Wang, Zeng-Yu
Chai, Maofeng
author_facet Sun, Yue
Li, He
Wu, Jiajing
Zhang, Kangning
Tang, Wei
Cong, Lili
Xie, Hongli
Wang, Zeng-Yu
Chai, Maofeng
author_sort Sun, Yue
collection PubMed
description Growth-regulating factors (GRFs) play crucial roles in plant growth and stress response. To date, there have been no reports of the analysis and identification of the GRF transcription factor family in alfalfa. In this study, we identified 27 GRF family members from alfalfa (Medicago sativa L.) “Xinjiang Daye”, and analyzed their physicochemical properties. Based on phylogenetic analysis, these MsGRFs were divided into five subgroups, each with a similar gene structure and conserved motifs. MsGRFs genes are distributed on 23 chromosomes, and all contain QLQ and WRC conserved domains. The results of the collinearity analysis showed that all MsGRFs are involved in gene duplication, including multiple whole-genome duplication or segmental duplication and a set of tandem duplication, indicating that large-scale duplication is important for the expansion of the GRF family in alfalfa. Several hormone-related and stress-related cis-acting elements have been found in the promoter regions of MsGRFs. Some MsGRFs were highly expressed in young leaves and stems, and their expression decreased during development. In addition, the leaf size of different varieties was found to vary, and MsGRF1 to 4, MsGRF18 to 20, and MsGRF22 to 23 were differentially expressed in large and small leaf alfalfa varieties, suggesting that they are critical in the regulation of leaf size. The results of this study can benefit further exploration of the regulatory functions of MsGRFs in growth and development, and can identify candidate genes that control leaf size development.
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spelling pubmed-94455732022-09-07 Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa Sun, Yue Li, He Wu, Jiajing Zhang, Kangning Tang, Wei Cong, Lili Xie, Hongli Wang, Zeng-Yu Chai, Maofeng Front Plant Sci Plant Science Growth-regulating factors (GRFs) play crucial roles in plant growth and stress response. To date, there have been no reports of the analysis and identification of the GRF transcription factor family in alfalfa. In this study, we identified 27 GRF family members from alfalfa (Medicago sativa L.) “Xinjiang Daye”, and analyzed their physicochemical properties. Based on phylogenetic analysis, these MsGRFs were divided into five subgroups, each with a similar gene structure and conserved motifs. MsGRFs genes are distributed on 23 chromosomes, and all contain QLQ and WRC conserved domains. The results of the collinearity analysis showed that all MsGRFs are involved in gene duplication, including multiple whole-genome duplication or segmental duplication and a set of tandem duplication, indicating that large-scale duplication is important for the expansion of the GRF family in alfalfa. Several hormone-related and stress-related cis-acting elements have been found in the promoter regions of MsGRFs. Some MsGRFs were highly expressed in young leaves and stems, and their expression decreased during development. In addition, the leaf size of different varieties was found to vary, and MsGRF1 to 4, MsGRF18 to 20, and MsGRF22 to 23 were differentially expressed in large and small leaf alfalfa varieties, suggesting that they are critical in the regulation of leaf size. The results of this study can benefit further exploration of the regulatory functions of MsGRFs in growth and development, and can identify candidate genes that control leaf size development. Frontiers Media S.A. 2022-08-23 /pmc/articles/PMC9445573/ /pubmed/36082290 http://dx.doi.org/10.3389/fpls.2022.964604 Text en Copyright © 2022 Sun, Li, Wu, Zhang, Tang, Cong, Xie, Wang and Chai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sun, Yue
Li, He
Wu, Jiajing
Zhang, Kangning
Tang, Wei
Cong, Lili
Xie, Hongli
Wang, Zeng-Yu
Chai, Maofeng
Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title_full Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title_fullStr Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title_full_unstemmed Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title_short Genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
title_sort genome-wide identification of growth-regulating factor transcription factor family related to leaf and stem development in alfalfa
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445573/
https://www.ncbi.nlm.nih.gov/pubmed/36082290
http://dx.doi.org/10.3389/fpls.2022.964604
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