Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection

The purpose of this study was to antibacterial, and antibiofilm activity of two Lactobacillus strains secretome and extraction against E. coli isolated from women with urinary tract infection (UTI). We isolated 100 E. coli samples from women with UTI. Lactobacillus acidophilus and Lactobacillus casei characteristics were evaluated, and their secretome and extraction were prepared. The antibacterial and antibiofilm activity of secretome and extraction of both Lactobacillus strains were evaluated against isolated E. coli samples. L. acidophilus and L. casei were able to tolerate pH 3, bile salts, and pancreatic enzymes. Both probiotics were not resistant to antibiotics and demonstrated an appropriate ability to adhere to the intestinal epithelial cells. Secretome and extraction of L. acidophilus and L. casei strains showed a good antibacterial and antibiofilm against E. coli isolates. Generally, present study suggested that the secretome and extraction of L. acidophilus and L. casei strains exhibits a good antimicrobial activity.