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Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices

Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for the quality of life and prognosis of people with cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity to inv...

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Autores principales: Cramer, Nina, Nawrot, Marie Luise, Wege, Lion, Dorda, Marie, Sommer, Charline, Danov, Olga, Wronski, Sabine, Braun, Armin, Jonigk, Danny, Fischer, Sebastian, Munder, Antje, Tümmler, Burkhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446154/
https://www.ncbi.nlm.nih.gov/pubmed/36081773
http://dx.doi.org/10.3389/fcimb.2022.992214
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author Cramer, Nina
Nawrot, Marie Luise
Wege, Lion
Dorda, Marie
Sommer, Charline
Danov, Olga
Wronski, Sabine
Braun, Armin
Jonigk, Danny
Fischer, Sebastian
Munder, Antje
Tümmler, Burkhard
author_facet Cramer, Nina
Nawrot, Marie Luise
Wege, Lion
Dorda, Marie
Sommer, Charline
Danov, Olga
Wronski, Sabine
Braun, Armin
Jonigk, Danny
Fischer, Sebastian
Munder, Antje
Tümmler, Burkhard
author_sort Cramer, Nina
collection PubMed
description Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for the quality of life and prognosis of people with cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity to investigate pathogen adaptation processes to the host. Our studies aimed to resolve if and to what extent the bacterial adaptation to the CF airways influences the fitness of the pathogen to grow and to persist in the lungs. Marker-free competitive fitness experiments of serial P. aeruginosa isolates differentiated by strain-specific SNPs, were performed with murine and human precision cut lung slices (PCLS). Serial P. aeruginosa isolates were selected from six mild and six severe CF patient courses, respectively. MPCLS or hPCLS were inoculated with a mixture of equal numbers of the serial isolates of one course. The temporal change of the composition of the bacterial community during competitive growth was quantified by multi-marker amplicon sequencing. Both ex vivo models displayed a strong separation of fitness traits between mild and severe courses. Whereas the earlier isolates dominated the competition in the severe courses, intermediate and late isolates commonly won the competition in the mild courses. The status of the CF lung disease rather than the bacterial genotype drives the adaptation of P. aeruginosa during chronic CF lung infection. This implies that the disease status of the lung habitat governed the adaptation of P. aeruginosa more strongly than the underlying bacterial clone-type and its genetic repertoire.
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spelling pubmed-94461542022-09-07 Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices Cramer, Nina Nawrot, Marie Luise Wege, Lion Dorda, Marie Sommer, Charline Danov, Olga Wronski, Sabine Braun, Armin Jonigk, Danny Fischer, Sebastian Munder, Antje Tümmler, Burkhard Front Cell Infect Microbiol Cellular and Infection Microbiology Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for the quality of life and prognosis of people with cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity to investigate pathogen adaptation processes to the host. Our studies aimed to resolve if and to what extent the bacterial adaptation to the CF airways influences the fitness of the pathogen to grow and to persist in the lungs. Marker-free competitive fitness experiments of serial P. aeruginosa isolates differentiated by strain-specific SNPs, were performed with murine and human precision cut lung slices (PCLS). Serial P. aeruginosa isolates were selected from six mild and six severe CF patient courses, respectively. MPCLS or hPCLS were inoculated with a mixture of equal numbers of the serial isolates of one course. The temporal change of the composition of the bacterial community during competitive growth was quantified by multi-marker amplicon sequencing. Both ex vivo models displayed a strong separation of fitness traits between mild and severe courses. Whereas the earlier isolates dominated the competition in the severe courses, intermediate and late isolates commonly won the competition in the mild courses. The status of the CF lung disease rather than the bacterial genotype drives the adaptation of P. aeruginosa during chronic CF lung infection. This implies that the disease status of the lung habitat governed the adaptation of P. aeruginosa more strongly than the underlying bacterial clone-type and its genetic repertoire. Frontiers Media S.A. 2022-08-23 /pmc/articles/PMC9446154/ /pubmed/36081773 http://dx.doi.org/10.3389/fcimb.2022.992214 Text en Copyright © 2022 Cramer, Nawrot, Wege, Dorda, Sommer, Danov, Wronski, Braun, Jonigk, Fischer, Munder and Tümmler https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Cramer, Nina
Nawrot, Marie Luise
Wege, Lion
Dorda, Marie
Sommer, Charline
Danov, Olga
Wronski, Sabine
Braun, Armin
Jonigk, Danny
Fischer, Sebastian
Munder, Antje
Tümmler, Burkhard
Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title_full Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title_fullStr Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title_full_unstemmed Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title_short Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
title_sort competitive fitness of pseudomonas aeruginosa isolates in human and murine precision-cut lung slices
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446154/
https://www.ncbi.nlm.nih.gov/pubmed/36081773
http://dx.doi.org/10.3389/fcimb.2022.992214
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