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Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility
Hyperactive sperm motility is important for successful fertilization. In the present study, a proteome profiling approach was performed to identify the differences between Landrace boars with different levels of hyperactive sperm motility in liquid extended semen. Two contrasts were studied: (i) hig...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446748/ https://www.ncbi.nlm.nih.gov/pubmed/36064611 http://dx.doi.org/10.1186/s13028-022-00642-1 |
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author | van Son, Maren Våge, Dag Inge Skaugen, Morten Tremoen, Nina Hårdnes Gaustad, Ann Helen Zeremichael, Teklu Tewoldebrhan Myromslien, Frøydis Deinboll Grindflek, Eli |
author_facet | van Son, Maren Våge, Dag Inge Skaugen, Morten Tremoen, Nina Hårdnes Gaustad, Ann Helen Zeremichael, Teklu Tewoldebrhan Myromslien, Frøydis Deinboll Grindflek, Eli |
author_sort | van Son, Maren |
collection | PubMed |
description | Hyperactive sperm motility is important for successful fertilization. In the present study, a proteome profiling approach was performed to identify the differences between Landrace boars with different levels of hyperactive sperm motility in liquid extended semen. Two contrasts were studied: (i) high versus low levels of sperm hyperactivity at semen collection day and (ii) high versus low change in levels of sperm hyperactivity after 96 h semen storage. Testicular samples were analyzed on a Q Exactive mass spectrometer and more than 6000 proteins were identified in the 13 samples. The most significant differentially expressed proteins were mediator complex subunit 28 (MED28), cell division cycle 37 like 1 (CDC37L1), ubiquitin specific peptidase 10 (USP10), zinc finger FYVE-type containing 26 (ZFYVE26), protein kinase C delta (PRKCD), actinin alpha 4 (ACTN4), N(alpha)-acetyltransferase 30 (NAA30), C1q domain-containing (LOC110258309) and uncharacterized LOC100512926. Of the differentially expressed proteins, 11 have previously been identified as differentially expressed at the corresponding mRNA transcript level using the same samples and contrasts. These include sphingosine kinase 1 isoform 2 (SPHK1), serine and arginine rich splicing factor 1 (SRSF1), and tubulin gamma-1 (TUBG1) which are involved in the acrosome reaction and sperm motility. A mass spectrometry approach was applied to investigate the protein profiles of boars with different levels of hyperactive sperm motility. This study identified several proteins previously shown to be involved in sperm motility and quality, but also proteins with no known function for sperm motility. Candidates that are differentially expressed on both mRNA and protein levels are especially relevant as biological markers of semen quality. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13028-022-00642-1. |
format | Online Article Text |
id | pubmed-9446748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94467482022-09-07 Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility van Son, Maren Våge, Dag Inge Skaugen, Morten Tremoen, Nina Hårdnes Gaustad, Ann Helen Zeremichael, Teklu Tewoldebrhan Myromslien, Frøydis Deinboll Grindflek, Eli Acta Vet Scand Brief Communication Hyperactive sperm motility is important for successful fertilization. In the present study, a proteome profiling approach was performed to identify the differences between Landrace boars with different levels of hyperactive sperm motility in liquid extended semen. Two contrasts were studied: (i) high versus low levels of sperm hyperactivity at semen collection day and (ii) high versus low change in levels of sperm hyperactivity after 96 h semen storage. Testicular samples were analyzed on a Q Exactive mass spectrometer and more than 6000 proteins were identified in the 13 samples. The most significant differentially expressed proteins were mediator complex subunit 28 (MED28), cell division cycle 37 like 1 (CDC37L1), ubiquitin specific peptidase 10 (USP10), zinc finger FYVE-type containing 26 (ZFYVE26), protein kinase C delta (PRKCD), actinin alpha 4 (ACTN4), N(alpha)-acetyltransferase 30 (NAA30), C1q domain-containing (LOC110258309) and uncharacterized LOC100512926. Of the differentially expressed proteins, 11 have previously been identified as differentially expressed at the corresponding mRNA transcript level using the same samples and contrasts. These include sphingosine kinase 1 isoform 2 (SPHK1), serine and arginine rich splicing factor 1 (SRSF1), and tubulin gamma-1 (TUBG1) which are involved in the acrosome reaction and sperm motility. A mass spectrometry approach was applied to investigate the protein profiles of boars with different levels of hyperactive sperm motility. This study identified several proteins previously shown to be involved in sperm motility and quality, but also proteins with no known function for sperm motility. Candidates that are differentially expressed on both mRNA and protein levels are especially relevant as biological markers of semen quality. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13028-022-00642-1. BioMed Central 2022-09-05 /pmc/articles/PMC9446748/ /pubmed/36064611 http://dx.doi.org/10.1186/s13028-022-00642-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Brief Communication van Son, Maren Våge, Dag Inge Skaugen, Morten Tremoen, Nina Hårdnes Gaustad, Ann Helen Zeremichael, Teklu Tewoldebrhan Myromslien, Frøydis Deinboll Grindflek, Eli Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title | Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title_full | Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title_fullStr | Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title_full_unstemmed | Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title_short | Protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
title_sort | protein profiling of testicular tissue from boars with different levels of hyperactive sperm motility |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446748/ https://www.ncbi.nlm.nih.gov/pubmed/36064611 http://dx.doi.org/10.1186/s13028-022-00642-1 |
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